Распространенность серотипов вируса денге у пациентов с геморрагической лихорадкой денге в Пешаваре (Пакистан)

Dengue virus (DENV) is categorized into 4 serotypes: DENV1, DENV2, DENV3, and DENV4. The objective of this study is to determine the association of DENV serotypes with dengue hemorrhagic fever (DHF) in DHF patients of Peshawar district, Pakistan. Material and methods. A total of 200 blood samples were collected from dengue fever (DF) and DHF patients. All of the samples were considered to be positive by NS1 ELISA. RNA was extracted and then converted into cDNA by using Revert AID Reverse transcriptase (Thermofisher scientific) the target region for transcription and Amplification is a c-prM region which was amplified by Regular PCR and by using Nested PCR, the DENV serotypes (1–4) was identified by using serotypes specific primers (TS1, TS2, TS3, and TS4) the samples was run through gel electrophoresis and then read the product size. Results. 164 (82%) samples were considered to be positive by both NS1 ELISA and real-time-PCR while 36 (18%) were negative by real-time-PCR. The study population consists of 60% male and 40% female. Within positive samples, 40 patients have DHF of which 24 (15%) were male and 16 (10%) were females. Most of the affected patients have an age range between 10–30 years followed by 30–40 years. All of the DHF patients were primary patients and had no previous dengue fever history. By using Serotype-specific primers all the DHF samples were positive for Serotype-2 (DENV2). This shows that DENV2 was primarily associated with DHF. Conclusion. DENV2 is considered to be prevalent serotype during dengue epidemic in District Peshawar, Khyberpakhtunkhwa, Pakistan.