TY - JOUR
T1 - A selective ion HPLC-APCI-MS method for the quantification of pentacyclic triterpenes in an anxiolytic botanical dietary supplement for the animal health market
AU - Liu, Rui
AU - Carballo-Arce, Ana Francis
AU - Singh, Ranpreet
AU - Saleem, Ammar
AU - Rocha, Marco
AU - Mullally, Martha
AU - Otarola-Rojas, Marco
AU - Alvarrez, Luis Poveda
AU - Sanchez-Vindas, Pablo
AU - Garcia, Mario
AU - Baker, John
AU - Merali, Zul
AU - Guerrero-Analco, Jose Antonio
AU - Durst, Tony
AU - Harris, Cory
AU - Arnason, John
N1 - Funding Information:
This work was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC). We thank Caño Palma Biological Station for assistance.
Publisher Copyright:
© 2019 Natural Product Incorporation. All rights reserved.
PY - 2019/1
Y1 - 2019/1
N2 - A new anti-anxiety dietary supplement was developed for the animal health market, by combining 2 triterpene containing botanicals, Souroubea sympetala (Marcgraviaceae) with Platanus occidentalis (Platanaceae) A validated method for a quality control of the botanical blend was achieved using HPLC-APCI-MS. The method resulted in the detection and quantitative determination of betulinic acid (1), and ursolic acid (2) in P. occidentalis and 1, 2, lupeol (3), β-amyrin (4) and α-amyrin (5) in S. sympetala and the finished product Zentrol TM . Detections were at low ng on column for 1 and 2 and in low µg range for 3, 4 and 5 using calibration curves within 10-100 ng (R 2 > 0.993). Recovery of spiked samples for all the recoveries observed were > 94%. Inter-day and intraday variations were 0.8-3.5% and 5-10.4%, respectively. These results indicate the suitability of the developed analytical method to detect and quantify triterpenes of raw materials used in the manufacture of natural health products.
AB - A new anti-anxiety dietary supplement was developed for the animal health market, by combining 2 triterpene containing botanicals, Souroubea sympetala (Marcgraviaceae) with Platanus occidentalis (Platanaceae) A validated method for a quality control of the botanical blend was achieved using HPLC-APCI-MS. The method resulted in the detection and quantitative determination of betulinic acid (1), and ursolic acid (2) in P. occidentalis and 1, 2, lupeol (3), β-amyrin (4) and α-amyrin (5) in S. sympetala and the finished product Zentrol TM . Detections were at low ng on column for 1 and 2 and in low µg range for 3, 4 and 5 using calibration curves within 10-100 ng (R 2 > 0.993). Recovery of spiked samples for all the recoveries observed were > 94%. Inter-day and intraday variations were 0.8-3.5% and 5-10.4%, respectively. These results indicate the suitability of the developed analytical method to detect and quantify triterpenes of raw materials used in the manufacture of natural health products.
KW - HPLC-APCI-MS
KW - Pentacyclic triterpenes
KW - Platanus
KW - Quality control
KW - Souroubea
UR - http://www.scopus.com/inward/record.url?scp=85063617063&partnerID=8YFLogxK
U2 - 10.1177/1934578X1901400104
DO - 10.1177/1934578X1901400104
M3 - Article
AN - SCOPUS:85063617063
SN - 1934-578X
VL - 14
SP - 11
EP - 14
JO - Natural Product Communications
JF - Natural Product Communications
IS - 1
ER -