Activity-guided isolation of a novel protein from Croton tiglium with antifungal and antibacterial activities

Muhammad Shahid; Muhammad Tayyab; Farah Naz; Amer Jamil; Muhammad Ashraf; Anwarul Hassan Gilani

doi:10.1002/ptr.2544

Activity-guided isolation of a novel protein from Croton tiglium with antifungal and antibacterial activities

Muhammad Shahid, Muhammad Tayyab, Farah Naz, Amer Jamil, Muhammad Ashraf, Anwarul Hassan Gilani

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

This study describes the activity-guided isolation and purification of a novel antimicrobial protein from the seed of Croton tiglium Linn. Purification was carried out by (NH₄)₂SO₄ precipitation, gel filtration and DEAE-cellulose ion-exchange chromatography. Antifungal and antibacterial activities were determined after each purification step. SDS-polyacrylamide gel electrophoresis revealed that the purified protein was a monomer with molecular mass of 50 kDa. This is a first report on purification of a protein from Croton tiglium, which possesses a strong and broad spectrum antimicrobial activity.

Original language	English
Pages (from-to)	1646-1649
Number of pages	4
Journal	Phytotherapy Research
Volume	22
Issue number	12
DOIs	https://doi.org/10.1002/ptr.2544
Publication status	Published - Dec 2008
Externally published	Yes

Keywords

Antibacterial
Antifungal
Bioactive peptide/protein
Croton tiglium

Access to Document

10.1002/ptr.2544

Cite this

@article{f13945ab7d4d41e087f0ff070acc20b0,

title = "Activity-guided isolation of a novel protein from Croton tiglium with antifungal and antibacterial activities",

abstract = "This study describes the activity-guided isolation and purification of a novel antimicrobial protein from the seed of Croton tiglium Linn. Purification was carried out by (NH4)2SO4 precipitation, gel filtration and DEAE-cellulose ion-exchange chromatography. Antifungal and antibacterial activities were determined after each purification step. SDS-polyacrylamide gel electrophoresis revealed that the purified protein was a monomer with molecular mass of 50 kDa. This is a first report on purification of a protein from Croton tiglium, which possesses a strong and broad spectrum antimicrobial activity.",

keywords = "Antibacterial, Antifungal, Bioactive peptide/protein, Croton tiglium",

author = "Muhammad Shahid and Muhammad Tayyab and Farah Naz and Amer Jamil and Muhammad Ashraf and Gilani, {Anwarul Hassan}",

year = "2008",

month = dec,

doi = "10.1002/ptr.2544",

language = "English",

volume = "22",

pages = "1646--1649",

journal = "Phytotherapy Research",

issn = "0951-418X",

publisher = "John Wiley and Sons Ltd",

number = "12",

}

TY - JOUR

T1 - Activity-guided isolation of a novel protein from Croton tiglium with antifungal and antibacterial activities

AU - Shahid, Muhammad

AU - Tayyab, Muhammad

AU - Naz, Farah

AU - Jamil, Amer

AU - Ashraf, Muhammad

AU - Gilani, Anwarul Hassan

PY - 2008/12

Y1 - 2008/12

N2 - This study describes the activity-guided isolation and purification of a novel antimicrobial protein from the seed of Croton tiglium Linn. Purification was carried out by (NH4)2SO4 precipitation, gel filtration and DEAE-cellulose ion-exchange chromatography. Antifungal and antibacterial activities were determined after each purification step. SDS-polyacrylamide gel electrophoresis revealed that the purified protein was a monomer with molecular mass of 50 kDa. This is a first report on purification of a protein from Croton tiglium, which possesses a strong and broad spectrum antimicrobial activity.

AB - This study describes the activity-guided isolation and purification of a novel antimicrobial protein from the seed of Croton tiglium Linn. Purification was carried out by (NH4)2SO4 precipitation, gel filtration and DEAE-cellulose ion-exchange chromatography. Antifungal and antibacterial activities were determined after each purification step. SDS-polyacrylamide gel electrophoresis revealed that the purified protein was a monomer with molecular mass of 50 kDa. This is a first report on purification of a protein from Croton tiglium, which possesses a strong and broad spectrum antimicrobial activity.

KW - Antibacterial

KW - Antifungal

KW - Bioactive peptide/protein

KW - Croton tiglium

UR - http://www.scopus.com/inward/record.url?scp=57549097481&partnerID=8YFLogxK

U2 - 10.1002/ptr.2544

DO - 10.1002/ptr.2544

M3 - Article

C2 - 18844289

AN - SCOPUS:57549097481

SN - 0951-418X

VL - 22

SP - 1646

EP - 1649

JO - Phytotherapy Research

JF - Phytotherapy Research

IS - 12

ER -

Activity-guided isolation of a novel protein from Croton tiglium with antifungal and antibacterial activities

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this