An endogenous inhibitor of Ca++-ATPase from human placenta

Masood U.L.Hassan Javed; Tahira Yasmeen Naru; Francesco Michelangeli

doi:10.1080/14756360009030348

An endogenous inhibitor of Ca⁺⁺-ATPase from human placenta

Masood U.L.Hassan Javed, Tahira Yasmeen Naru, Francesco Michelangeli

Department of Obstetrics & Gynaecology, Medical College, Pakistan

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Intracellular free calcium is regulated by Ca⁺⁺-ATPase, one form present on the plasma membrane (PM Ca⁺⁺-ATPase) and the other on sarcoplasmic (endoplasmic) reticulum (SR/ER Ca⁺⁺-ATPase). An endogenous inhibitor of SR Ca⁺⁺-ATPase from human placenta was shown to be present in normal placenta and the activity was not detectable in placenta from preeclamptic patients. The inhibitor was distributed in cytosol and microsomes. The inhibition of Ca⁺⁺-ATPase by this inhibitor was concentration- and time-dependent. The inhibitor neither bound to DEAE- nor CM-sepharose resins at pH 7.5 and 8.5. Furthermore, it was heat stable for 15 min up to 55°C and completely destroyed at 80°C in a few minutes. It was also observed to be stable at room temperature for at least 3 months. The purification and characterization of this inhibitor would be valuable in achieving an understanding of the normal regulation of Ca⁺⁺-ATPase in the placenta during pregnancy.

Original language	English (US)
Pages (from-to)	163-170
Number of pages	8
Journal	Journal of Enzyme Inhibition
Volume	15
Issue number	2
DOIs	https://doi.org/10.1080/14756360009030348
Publication status	Published - 2000

Keywords

Ca-ATPase
Inhibitor
Placenta

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10.1080/14756360009030348

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title = "An endogenous inhibitor of Ca++-ATPase from human placenta",

abstract = "Intracellular free calcium is regulated by Ca++-ATPase, one form present on the plasma membrane (PM Ca++-ATPase) and the other on sarcoplasmic (endoplasmic) reticulum (SR/ER Ca++-ATPase). An endogenous inhibitor of SR Ca++-ATPase from human placenta was shown to be present in normal placenta and the activity was not detectable in placenta from preeclamptic patients. The inhibitor was distributed in cytosol and microsomes. The inhibition of Ca++-ATPase by this inhibitor was concentration- and time-dependent. The inhibitor neither bound to DEAE- nor CM-sepharose resins at pH 7.5 and 8.5. Furthermore, it was heat stable for 15 min up to 55°C and completely destroyed at 80°C in a few minutes. It was also observed to be stable at room temperature for at least 3 months. The purification and characterization of this inhibitor would be valuable in achieving an understanding of the normal regulation of Ca++-ATPase in the placenta during pregnancy.",

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AU - Javed, Masood U.L.Hassan

AU - Naru, Tahira Yasmeen

AU - Michelangeli, Francesco

PY - 2000

Y1 - 2000

N2 - Intracellular free calcium is regulated by Ca++-ATPase, one form present on the plasma membrane (PM Ca++-ATPase) and the other on sarcoplasmic (endoplasmic) reticulum (SR/ER Ca++-ATPase). An endogenous inhibitor of SR Ca++-ATPase from human placenta was shown to be present in normal placenta and the activity was not detectable in placenta from preeclamptic patients. The inhibitor was distributed in cytosol and microsomes. The inhibition of Ca++-ATPase by this inhibitor was concentration- and time-dependent. The inhibitor neither bound to DEAE- nor CM-sepharose resins at pH 7.5 and 8.5. Furthermore, it was heat stable for 15 min up to 55°C and completely destroyed at 80°C in a few minutes. It was also observed to be stable at room temperature for at least 3 months. The purification and characterization of this inhibitor would be valuable in achieving an understanding of the normal regulation of Ca++-ATPase in the placenta during pregnancy.

AB - Intracellular free calcium is regulated by Ca++-ATPase, one form present on the plasma membrane (PM Ca++-ATPase) and the other on sarcoplasmic (endoplasmic) reticulum (SR/ER Ca++-ATPase). An endogenous inhibitor of SR Ca++-ATPase from human placenta was shown to be present in normal placenta and the activity was not detectable in placenta from preeclamptic patients. The inhibitor was distributed in cytosol and microsomes. The inhibition of Ca++-ATPase by this inhibitor was concentration- and time-dependent. The inhibitor neither bound to DEAE- nor CM-sepharose resins at pH 7.5 and 8.5. Furthermore, it was heat stable for 15 min up to 55°C and completely destroyed at 80°C in a few minutes. It was also observed to be stable at room temperature for at least 3 months. The purification and characterization of this inhibitor would be valuable in achieving an understanding of the normal regulation of Ca++-ATPase in the placenta during pregnancy.

KW - Ca-ATPase

KW - Inhibitor

KW - Placenta

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An endogenous inhibitor of Ca⁺⁺-ATPase from human placenta

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