A competitive enzyme-linked immunosorbent assay (ELISA) has been developed for the cytokine, granulocyte-macrophage colony stimulating factor (GM-CSF) using polyclonal antibody. The sensitivity of the assay system approximates 20 pg of immunoreactive cytokine protein. No cross-reactivity was found with human granulocyte-colony stimulating factor. Mean coefficient of variation for between-assay precision was found to be 17%. The assay is rapid, sensitive, reliable and quite suitable to study the levels of GM-CSF in biological fluids. It was used to find out the serum levels of GM-CSF in patients with myocardial infarction, neutropenic cancer patients and in normal healthy subjects. No statistically significant differences were found in the mean serum levels of GM-CSF in these three groups.
|Number of pages||6|
|Journal||Pakistan Journal of Zoology|
|Publication status||Published - 2002|
- Enzyme-linked immunosorbent assay
- Granulocyte-macrophage colony stimulating factor
- Myocardial infarction