Background: Evidence shows that Kangaroo Mother Care (KMC) can prevent up to half of all deaths in low-birthweight (LBW) babies compared with incubator care but implementation in Uganda is still poor. Objectives: To explore the healthcare workers’ knowledge on KMC in caring for low-birthweight babies. Study site: A large Neonatal Intensive Care Unit (NICU) in Uganda. Study participants: All staff working in NICU. Design: A quantitative approach was used. Results: Only 12% of the participants could define KMC and 21% thought it would cause asphyxia with most saying it was too time consuming. Conclusion and recommendations: Further education is needed for NICU staff.