TY - JOUR
T1 - An improved method for processing early rat otocysts for the electron microscope.
AU - Schulte, T.
AU - Khan, K. M.
AU - Marovitz, W. F.
PY - 1977/1
Y1 - 1977/1
N2 - Various methods of dissection, fixation, osmication, sectioning and staining were tested in order to develop an acceptable technique for preparing 9, 10, 11, and 12-day-old rat otocysts for electron microscopic study. The general problems associated with embryonic tissue-difficult handling, high water content and poor stainability are discussed, and concrete methods of preparation which significantly decrease these difficulties are proposed. The specific fixation and sectioning requirements of rat otocyst are also described in the elaboration of a method which will be used in subsequent studies of the organogenesis of rodent ear.
AB - Various methods of dissection, fixation, osmication, sectioning and staining were tested in order to develop an acceptable technique for preparing 9, 10, 11, and 12-day-old rat otocysts for electron microscopic study. The general problems associated with embryonic tissue-difficult handling, high water content and poor stainability are discussed, and concrete methods of preparation which significantly decrease these difficulties are proposed. The specific fixation and sectioning requirements of rat otocyst are also described in the elaboration of a method which will be used in subsequent studies of the organogenesis of rodent ear.
UR - http://www.scopus.com/inward/record.url?scp=0017440991&partnerID=8YFLogxK
M3 - Article
C2 - 65936
AN - SCOPUS:0017440991
SN - 0096-8056
VL - 86
SP - 1
EP - 8
JO - The Annals of otology, rhinology & laryngology. Supplement
JF - The Annals of otology, rhinology & laryngology. Supplement
IS - 1 Pt 2 Suppl 35
ER -