Antithyroid effects of diphenyl hydantoin sodium

Objective to confirm antithyroid effects of Diphenyl hydantoin (DPH) and determine whether they are reversible by the administration of putrescine in rats. Methods A randomized control trial was carried out during a period of four weeks on 30 female rats weighing 248 to 320 grams. They were divided into three groups each comprised of ten rats; Group I (control) was treated with normal saline, Group II (DPH treated) and Group III (treated with DPH and Putrescine). After the treatment, blood was collected by cardiac puncture on 18th day and radioimmunoassay was performed to measure Serum T3 (triiodo thyronine), T4 (Thyroxine) and TSH (Thyroid stimulating hormone) levels in all groups. Results were considered to be significant at p value<0.05. Results In group II there was a fall in T3, T4 concentration with significant rise in TSH concentration as compared to the control group. The combined administration of putrescine and DPH resulted in a rise of serum T3 and T4 with negligible fall in TSH; results were not significant. Conclusion We found DPH induced hypo-thyrodism improved but did not reverse by the administration of Putrescine. Keeping in mind anti thyroid effects of DPH, this anticonvulsant drug should be given with careful monitoring of thyroid status.