Aspergillus-specific IgG antibodies for diagnosing chronic pulmonary aspergillosis compared to the reference standard

for the CPAnet

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4 Citations (Scopus)

Abstract

Objectives: To evaluate the performance of Aspergillus-specific IgG antibodies for diagnosing chronic pulmonary aspergillosis (CPA) by using a cohort of patients with histologically proven CPA as a reference standard. Methods: We collected Aspergillus-specific IgG antibody titres from patients with histologically proven CPA in collaboration with CPAnet study sites in Denmark, Germany, Belgium, India, Moldova, and Pakistan (N = 47). Additionally, sera from diseased and healthy controls were prospectively collected at the Medical Clinic of the Research Center, Borstel, Germany (n = 303). Aspergillus-specific IgG antibody titres were measured by the ImmunoCAP® assay (Phadia 100, Thermo Fisher Scientific, Uppsala, Sweden). An Aspergillus-specific IgG antibody titre ≥50 mgA/L was considered positive. Results: Using patients with histologically proven CPA as the reference standard, the ImmunoCAP® Aspergillus-specific IgG antibody test had a sensitivity and specificity of 85.1% (95% CI: 71.7–93.8%) and 83.6% (95% CI: 78.0–88.3%), respectively. Patients with histologically proven CPA had significantly higher Aspergillus-specific IgG antibody titre with a median of 83.45 mgA/L (interquartile range 38.9–115.5) than all other cohorts (p < 0.001). False-positive test results occurred in one-third of 79 healthy controls. Discussion: Our study results confirm a high sensitivity of the Aspergillus-specific IgG antibody test for the diagnosis of CPA when using patients with histologically proven CPA as a reference standard. However, positive test results should always match radiological findings as false-positive test results limit the interpretation of the test.

Original languageEnglish
Pages (from-to)1605.e1-1605.e4
JournalClinical Microbiology and Infection
Volume29
Issue number12
DOIs
Publication statusPublished - Dec 2023

Keywords

  • Aspergillus-specific IgG antibody
  • CPA
  • Reference standard

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