Assessment of immunity to mycobacterial infection with luciferase reporter constructs

Valerie A. Snewin, Marie Pierre Gares, Peadar Ó Gaora, Zahra Hasan, Ivor N. Brown, Douglas B. Young

Research output: Contribution to journalArticlepeer-review

159 Citations (Scopus)

Abstract

Protective immunity to mycobacterial infection is incompletely understood but probably involves the coordinated interaction of multiple cell types and cytokines. With the aim of developing assays that might provide a surrogate measure of protective immunity, we have investigated the use of recombinant mycobacteria carrying luciferase reporter enzymes to assess the effectiveness of antimycobacterial immunity in model systems. Measurement of luminescence was shown to provide a rapid and simple alternative to the counting of CFU as a means of monitoring mycobacterial viability. We describe optimization of a luciferase reporter strain of Mycobacterium tuberculosis and demonstrate its application for the study of mycobacterial interactions with host cells in tissue culture and the rapid assessment of vaccine efficacy in a murine model.

Original languageEnglish
Pages (from-to)4586-4593
Number of pages8
JournalInfection and Immunity
Volume67
Issue number9
DOIs
Publication statusPublished - 1999

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