Benzamide sulfonamide derivatives: Potent inhibitors of carbonic anhydrase-II

Muhammad Saleem, Aamer Saeed, Atia-Tul-Wahab, Ajmal Khan, Sanaullah Abbasi, Waqasuddin Khan, Sher Bahadar Khan, M. Iqbal Choudhary

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Benzamide sulfonamide and its derivatives were identified as potent inhibitors of carbonic anhydrase-II (bovine carbonic anhydrase-II (bCA-II) and human recombinant carbonic anhydrase-II (hCA-II)) with IC50 values 0.06-0.28 μM and 0.09-0.58 μM, respectively. Different kinetics parameter, such as V max, K m, and K i, were determined. Molecular docking simulation studies on the lead compounds were carried out by AutoDock Vina docking software. The compounds were also evaluated for their cytotoxicity against 3T3 (Normal Cell Lines Mouse Fibroblast) cell line. All the compounds have shown non-cytotoxic effect toward 3T3 cell lines. This study has identified novel scaffolds for further hit-to-lead optimization for the discovery of effective drugs against carbonic anhydrase-II-associated disorder, such as glaucoma, leukemia, cystic fibrosis, and epilepsy.

Original languageEnglish
Pages (from-to)438-448
Number of pages11
JournalMedicinal Chemistry Research
Issue number3
Publication statusPublished - 1 Mar 2016
Externally publishedYes


  • 3T3 cell lines
  • AutoDock Vina docking software
  • Carbonic anhydrase-II
  • Cystic fibrosis
  • Epilepsy
  • Glaucoma
  • Grafit Version 7 software
  • IC
  • K
  • Leukemia
  • Sulfanilamide
  • Thiourea
  • V


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