Biodecolorization of reactive black-5 by a metal and salt tolerant bacterial strain Pseudomonas sp. RA20 isolated from Paharang drain effluents in Pakistan

Sabir Hussain, Zahid Maqbool, Shafaqat Ali, Tahira Yasmeen, Muhammad Imran, Faisal Mahmood, Farhat Abbas

Research output: Contribution to journalArticlepeer-review

68 Citations (Scopus)

Abstract

Discharge of untreated azo dyes contaminated textile wastewater into soil and water bodies causes severe contamination. The present study was conducted to isolate dye degrading bacterial strains from a textile industry wastewater carrying drain in the neighborhood of Faisalabad, Pakistan. Seventy six bacterial strains were initially isolated and was screened using liquid mineral salts medium spiked with Reactive Black-5 azo dye. The strain RA20 was found to be the most efficient azo dye degrading bacterial isolate and was identified by amplifying and sequencing its 16S rRNA. Analysis indicated that this strain belonged to genus Pseudomonas and was designated as Pseudomonas sp. RA20. It had the highest decolorization activity at pH 8 and 25°C incubation temperature under static conditions using yeast extract as an additional C source. This strain was also effective in decolorizing structurally related other reactive dyes including Reactive Orange 16, Reactive Yellow 2 and Reactive Red 120 but with varying efficacy. RA20 decolorized Reactive Black-5 significantly in the presence of up to 30gL-1 NaCl; however, the decolorization rate was significantly (p≤0.05) reduced beyond this salt concentration. Moreover, this bacterial strain also exhibited moderate tolerance to different heavy metals including zinc (Zn), cadmium (Cd), chromium (Cr), lead (Pb) and copper (Cu). RA20 also decolorized Reactive Black-5 in the presence of a mixture of the selected heavy metals depending upon their concentrations. This study highlights the importance of Pseudomonas sp. RA20 as a prospective biological resource for bioremediation of water and soils contaminated with azo dyes.

Original languageEnglish
Pages (from-to)331-338
Number of pages8
JournalEcotoxicology and Environmental Safety
Volume98
DOIs
Publication statusPublished - 1 Dec 2013
Externally publishedYes

Keywords

  • Decolorization
  • Pseudomonas sp. RA20
  • Recative dyes
  • Salt and heavy metal tolerance

Fingerprint

Dive into the research topics of 'Biodecolorization of reactive black-5 by a metal and salt tolerant bacterial strain Pseudomonas sp. RA20 isolated from Paharang drain effluents in Pakistan'. Together they form a unique fingerprint.

Cite this