Bortezomib-mediated expression of p27Kip1 through S-phase kinase protein 2 degradation in epithelial ovarian cancer

Shahabuddin; Maqbool Ahmed; Azhar Hussain; Zeenath Jehan; Fouad Al-Dayel; Adnan Munkarah; Prashant Bavi; Khawla S Al-Kuraya

Bortezomib-mediated expression of p27Kip1 through S-phase kinase protein 2 degradation in epithelial ovarian cancer

Shahabuddin
, Maqbool Ahmed
, Azhar Hussain
, Zeenath Jehan
, Fouad Al-Dayel
, Adnan Munkarah
, Prashant Bavi
, Khawla S Al-Kuraya

Department of Biological & Biomedical Sciences, Medical College, Pakistan

Research output: Contribution to journal › Article

Abstract

S-phase kinase protein 2 (SKP2), an F-box protein, targets cell-cycle regulators including cyclin-dependent kinase inhibitor p27Kip1 through ubiquitin-mediated degradation. SKP2 is frequently overexpressed in variety of cancers. We investigated the function of SKP2 and its ubiquitin–proteasome pathway in a large series (156) of epithelial ovarian cancer (EOC) patient samples, using a panel of cell lines, and nude mouse model. Using immunohistochemistry, we detected SKP2 in 13.2% tumor samples and found that it was inversely associated with p27Kip1. EOC subset with high level of SKP2 and low level of p27Kip1 showed a strong association with proliferative marker Ki167 (P

Original language	Undefined/Unknown
Journal	Laboratory Investigation
Publication status	Published - 1 Jan 2009

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access to Document

http://ecommons.aku.edu/crm/49

Cite this

@article{7c014232ce794587813298e132e51365,

title = "Bortezomib-mediated expression of p27Kip1 through S-phase kinase protein 2 degradation in epithelial ovarian cancer",

abstract = "S-phase kinase protein 2 (SKP2), an F-box protein, targets cell-cycle regulators including cyclin-dependent kinase inhibitor p27Kip1 through ubiquitin-mediated degradation. SKP2 is frequently overexpressed in variety of cancers. We investigated the function of SKP2 and its ubiquitin–proteasome pathway in a large series (156) of epithelial ovarian cancer (EOC) patient samples, using a panel of cell lines, and nude mouse model. Using immunohistochemistry, we detected SKP2 in 13.2\% tumor samples and found that it was inversely associated with p27Kip1. EOC subset with high level of SKP2 and low level of p27Kip1 showed a strong association with proliferative marker Ki167 (P ",

author = "Shahabuddin and Maqbool Ahmed and Azhar Hussain and Zeenath Jehan and Fouad Al-Dayel and Adnan Munkarah and Prashant Bavi and Al-Kuraya, \{Khawla S\}",

year = "2009",

month = jan,

day = "1",

language = "Undefined/Unknown",

journal = "Laboratory Investigation",

issn = "0023-6837",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - Bortezomib-mediated expression of p27Kip1 through S-phase kinase protein 2 degradation in epithelial ovarian cancer

AU - Shahabuddin,

AU - Ahmed, Maqbool

AU - Hussain, Azhar

AU - Jehan, Zeenath

AU - Al-Dayel, Fouad

AU - Munkarah, Adnan

AU - Bavi, Prashant

AU - Al-Kuraya, Khawla S

PY - 2009/1/1

Y1 - 2009/1/1

N2 - S-phase kinase protein 2 (SKP2), an F-box protein, targets cell-cycle regulators including cyclin-dependent kinase inhibitor p27Kip1 through ubiquitin-mediated degradation. SKP2 is frequently overexpressed in variety of cancers. We investigated the function of SKP2 and its ubiquitin–proteasome pathway in a large series (156) of epithelial ovarian cancer (EOC) patient samples, using a panel of cell lines, and nude mouse model. Using immunohistochemistry, we detected SKP2 in 13.2% tumor samples and found that it was inversely associated with p27Kip1. EOC subset with high level of SKP2 and low level of p27Kip1 showed a strong association with proliferative marker Ki167 (P

AB - S-phase kinase protein 2 (SKP2), an F-box protein, targets cell-cycle regulators including cyclin-dependent kinase inhibitor p27Kip1 through ubiquitin-mediated degradation. SKP2 is frequently overexpressed in variety of cancers. We investigated the function of SKP2 and its ubiquitin–proteasome pathway in a large series (156) of epithelial ovarian cancer (EOC) patient samples, using a panel of cell lines, and nude mouse model. Using immunohistochemistry, we detected SKP2 in 13.2% tumor samples and found that it was inversely associated with p27Kip1. EOC subset with high level of SKP2 and low level of p27Kip1 showed a strong association with proliferative marker Ki167 (P

M3 - Article

SN - 0023-6837

JO - Laboratory Investigation

JF - Laboratory Investigation

ER -