Carbofuran-induced effects on acetylcholinesterase (AChE) in erythrocytes and liver of Cyprinus carpio

Shahid Mahboob, K. A. Al-Ghanim, H. A. Fares Al-Balwi, F. Al-Misned, Z. Ahmad

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


A study was conducted to determine the effects of long-term exposure to carbofuran on erythrocyte and liver acetylcholinesterase (AChE) activity in Cyprinus carpio, and assess which tissue was more sensitive to pesticide exposure examining this enzyme. Fish were allowed to accilimatize in toxicant-free water for 24 days after 60-day exposure. AChE activity was determined with a spectrophotometer using acetylthiocholine iodide as a substrate in erythrocytes and liver. Enzymic activity of erythrocytes and liver decreased over time. A higher degree of enzyme inhibition was observed in erythrocyte compared to liver. The degree of enzyme inhibition was positively correlated with exposure time. When exposed fish were transferred to clean water, recovery was greater in erythrocytes than liver. Erythrocyte AChE activity recovered after 18 days, while it required 21 days in liver. The findings of this study indicate that erythrocyte AChE is more sensitive to carbofuran than liver. The greater sensitivity of erythrocyte AChE suggests that it may be more useful as a biomarker for monitoring status of pollution in freshwater bodies.

Original languageEnglish
Pages (from-to)614-623
Number of pages10
JournalToxicological and Environmental Chemistry
Issue number4
Publication statusPublished - 21 Apr 2014
Externally publishedYes


  • Cyprinus carpio
  • acetylcholinesterase
  • erythrocyte
  • liver
  • sensitivity


Dive into the research topics of 'Carbofuran-induced effects on acetylcholinesterase (AChE) in erythrocytes and liver of Cyprinus carpio'. Together they form a unique fingerprint.

Cite this