Chikungunya outbreak in Karachi Pakistan 2016-2017: An analysis of viral isolates

Dhani Prakoso, Kelli Barr, Kehkashan Imtiaz, Joveria Farooqi, Erum Khan, Maureen Long

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1 Citation (Scopus)


In December 2016 physicians in Karachi, Pakistan,witnessed an increase in patients presenting with febrile illness and severe polyarthralgia. Subsequently, chikungunya virus (CHIKV)) was isolated from three patients. This virus was sequenced and compared with other isolates of CHIKV obtained in India and Pakistan during recent outbreaks. Phylogenetic analysis indicated that the Karachi isolates were most similar to the East Central South African CHIKV lineage and showed sequence homology to isolates obtained in other parts of Pakistan and India. More importantly, two of the CHIKV isolates had a nucleotide substitution in the E1 gene corresponding to an amino acid change at chain F portion of the E1 protein.

Original languageEnglish
Pages (from-to)1467-1471
Number of pages5
JournalJournal of the Pakistan Medical Association
Issue number5
Publication statusPublished - May 2021


  • Arbovirus
  • Chikungunya virus
  • Karachi-Sindh
  • Nucleotide sequence
  • Pakistan


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