Chikungunya outbreak in Karachi Pakistan 2016-2017: An analysis of viral isolates

Dhani Prakoso; Kelli Barr; Kehkashan Imtiaz; Joveria Farooqi; Erum Khan; Maureen Long

doi:10.47391/JPMA.1287

Chikungunya outbreak in Karachi Pakistan 2016-2017: An analysis of viral isolates

Dhani Prakoso, Kelli Barr, Kehkashan Imtiaz, Joveria Farooqi, Erum Khan, Maureen Long

Department of Pathology and Laboratory Medicine, Medical College, Pakistan

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

In December 2016 physicians in Karachi, Pakistan,witnessed an increase in patients presenting with febrile illness and severe polyarthralgia. Subsequently, chikungunya virus (CHIKV)) was isolated from three patients. This virus was sequenced and compared with other isolates of CHIKV obtained in India and Pakistan during recent outbreaks. Phylogenetic analysis indicated that the Karachi isolates were most similar to the East Central South African CHIKV lineage and showed sequence homology to isolates obtained in other parts of Pakistan and India. More importantly, two of the CHIKV isolates had a nucleotide substitution in the E1 gene corresponding to an amino acid change at chain F portion of the E1 protein.

Original language	English
Pages (from-to)	1467-1471
Number of pages	5
Journal	Journal of the Pakistan Medical Association
Volume	71
Issue number	5
DOIs	https://doi.org/10.47391/JPMA.1287
Publication status	Published - May 2021

Keywords

Arbovirus
Chikungunya virus
Karachi-Sindh
Nucleotide sequence
Pakistan

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title = "Chikungunya outbreak in Karachi Pakistan 2016-2017: An analysis of viral isolates",

abstract = "In December 2016 physicians in Karachi, Pakistan,witnessed an increase in patients presenting with febrile illness and severe polyarthralgia. Subsequently, chikungunya virus (CHIKV)) was isolated from three patients. This virus was sequenced and compared with other isolates of CHIKV obtained in India and Pakistan during recent outbreaks. Phylogenetic analysis indicated that the Karachi isolates were most similar to the East Central South African CHIKV lineage and showed sequence homology to isolates obtained in other parts of Pakistan and India. More importantly, two of the CHIKV isolates had a nucleotide substitution in the E1 gene corresponding to an amino acid change at chain F portion of the E1 protein.",

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T1 - Chikungunya outbreak in Karachi Pakistan 2016-2017

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AU - Prakoso, Dhani

AU - Barr, Kelli

AU - Imtiaz, Kehkashan

AU - Farooqi, Joveria

AU - Khan, Erum

AU - Long, Maureen

PY - 2021/5

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AB - In December 2016 physicians in Karachi, Pakistan,witnessed an increase in patients presenting with febrile illness and severe polyarthralgia. Subsequently, chikungunya virus (CHIKV)) was isolated from three patients. This virus was sequenced and compared with other isolates of CHIKV obtained in India and Pakistan during recent outbreaks. Phylogenetic analysis indicated that the Karachi isolates were most similar to the East Central South African CHIKV lineage and showed sequence homology to isolates obtained in other parts of Pakistan and India. More importantly, two of the CHIKV isolates had a nucleotide substitution in the E1 gene corresponding to an amino acid change at chain F portion of the E1 protein.

KW - Arbovirus

KW - Chikungunya virus

KW - Karachi-Sindh

KW - Nucleotide sequence

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Chikungunya outbreak in Karachi Pakistan 2016-2017: An analysis of viral isolates

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