Citric acid enhances the phytoextraction of chromium, plant growth, and photosynthesis by alleviating the oxidative damages in Brassica napus L.

Sehar Afshan, Shafaqat Ali, Saima Aslam Bharwana, Muhammad Rizwan, Mujahid Farid, Farhat Abbas, Muhammad Ibrahim, Muhammad Aamer Mehmood, Ghulam Hasan Abbasi

Research output: Contribution to journalArticlepeer-review

184 Citations (Scopus)

Abstract

Chromium (Cr) toxicity is widespread in crops grown on Cr-contaminated soils and has become a serious environmental issue which requires affordable strategies for the remediation of such soils. This study was performed to assess the performance of citric acid (CA) through growing Brassica napus in the phytoextraction of Cr from contaminated soil. Different Cr (0, 100, and 500 μM) and citric acid (0, 2.5, and 5.0 mM) treatments were applied alone and in combinations to 4-week-old seedlings of B. napus plants in soil under wire house condition. Plants were harvested after 12 weeks of sowing, and the data was recorded regarding growth characteristics, biomass, photosynthetic pigments, malondialdehyde (MDA), electrolytic leakage (EL), antioxidant enzymes, and Cr uptake and accumulation. The results showed that the plant growth, biomass, chlorophyll contents, and carotenoid as well as soluble protein concentrations significantly decreased under Cr stress alone while these adverse effects were alleviated by application of CA. Cr concentration in roots, stem, and leaves of CA-supplied plant was significantly reduced while total uptake of Cr increased in all plant parts with CA application. Furthermore, in comparison with Cr treatments alone, CA supply reduced the MDA and EL values in both shoots and roots. Moreover, the activity of superoxide dismutase (SOD), guaiacol peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) in shoots and roots markedly increased by 100 μM Cr exposure, while decreased at 500 μM Cr stress. CA application enhanced the activities of antioxidant enzymes compared to the same Cr treatment alone. Thus, the data indicate that exogenous CA application can increase Cr uptake and can minimize Cr stress in plants and may be beneficial in accelerating the phytoextraction of Cr through hyper-accumulating plants such as B. napus.

Original languageEnglish
Pages (from-to)11679-11689
Number of pages11
JournalEnvironmental Science and Pollution Research
Volume22
Issue number15
DOIs
Publication statusPublished - 25 Aug 2015
Externally publishedYes

Keywords

  • Biomass
  • Brassica napus
  • Chromium
  • Citric acid
  • Electrolyte leakage
  • Guaiacol peroxidase
  • Phytoextractions

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