TY - JOUR
T1 - Cross talk between serum Kisspeptin-leptin during assisted reproduction techniques
AU - Rehman, Rehana
AU - Jamil, Zehra
AU - Khalid, Aqsa
AU - Fatima, Syeda Sadia
N1 - Funding Information:
The researchers wish to thank Dr. Syed Sajjad Hussain (CEO, Australian Concept Infertility Medical Centre). We are also grateful to all our study participants for their support and Ms. Aaida Mumtaz for assisting us in the data collection and management. This work was supported by Aga Khan University (Seed Money Grant # PF-59/0914) and Department of Biological and Biomedical Sciences start-up funds Conflict of interest: The authors declare that we have no conflict of interest.
Publisher Copyright:
© 2018, Professional Medical Publications. All rights reserved.
PY - 2018/3/1
Y1 - 2018/3/1
N2 - Background & objective: Leptin facilitates onset of puberty by impact on hypothalamic Kisspeptin, gonadotropin releasing hormone, follicle stimulating and luteinizing hormone. The link of peripheral Leptin-Kisspeptin in regulating the ovarian and endometrial tissue in relation to adiposity is unknown. Therefore, we wanted to identify Kisspeptin-Leptin association with body mass index (BMI) and success of assisted reproductive treatments (ART) in infertile females. Methods: A cross sectional study was carried from August 2015 till May 2017 after receiving ethical approval at Australian Concept Infertility Medical Centre, and Aga Khan University. The study group comprised of females with an age range of 25-37 year who had duration of unexplained infertility for more than two years. They were grouped as; underweight (<18 kg/m2), normal weight (18-22.9 kg/m2), overweight 23-24.99 kg/m2 and obese (>25 kg/m2). Kisspeptin and Leptin levels were measured by enzyme linked immune sorbent assay before down regulation of ovaries and initiation of treatment protocol of ART.Failure of procedure was detected by beta human chorionic gonadotropin <25mIU/ml (non-pregnant) whereas females with levels >25mIU/ml and cardiac activity on trans-vaginal scan were declared pregnant. Results: Highest Kisspeptin and Leptin levels were seen in normal weight group (374.80 ± 185.08ng/L; 12.78 ± 6.8 pg/ml) respectively, yet the highest number of clinical pregnancy was observed in overweight group (42%).A strong correlation of Kisspeptin with Leptin (r=0.794, p=0.001) was observed in the overweight females. Conclusion: Leptin-Kisspeptin-fertility link is expressed by maximum number of clinical pregnancies in the female group that showed strongest relationship between serum Leptin and Kisspeptin levels, irrespective of their BMI.
AB - Background & objective: Leptin facilitates onset of puberty by impact on hypothalamic Kisspeptin, gonadotropin releasing hormone, follicle stimulating and luteinizing hormone. The link of peripheral Leptin-Kisspeptin in regulating the ovarian and endometrial tissue in relation to adiposity is unknown. Therefore, we wanted to identify Kisspeptin-Leptin association with body mass index (BMI) and success of assisted reproductive treatments (ART) in infertile females. Methods: A cross sectional study was carried from August 2015 till May 2017 after receiving ethical approval at Australian Concept Infertility Medical Centre, and Aga Khan University. The study group comprised of females with an age range of 25-37 year who had duration of unexplained infertility for more than two years. They were grouped as; underweight (<18 kg/m2), normal weight (18-22.9 kg/m2), overweight 23-24.99 kg/m2 and obese (>25 kg/m2). Kisspeptin and Leptin levels were measured by enzyme linked immune sorbent assay before down regulation of ovaries and initiation of treatment protocol of ART.Failure of procedure was detected by beta human chorionic gonadotropin <25mIU/ml (non-pregnant) whereas females with levels >25mIU/ml and cardiac activity on trans-vaginal scan were declared pregnant. Results: Highest Kisspeptin and Leptin levels were seen in normal weight group (374.80 ± 185.08ng/L; 12.78 ± 6.8 pg/ml) respectively, yet the highest number of clinical pregnancy was observed in overweight group (42%).A strong correlation of Kisspeptin with Leptin (r=0.794, p=0.001) was observed in the overweight females. Conclusion: Leptin-Kisspeptin-fertility link is expressed by maximum number of clinical pregnancies in the female group that showed strongest relationship between serum Leptin and Kisspeptin levels, irrespective of their BMI.
KW - Assisted reproductive techniques
KW - Body mass index
KW - Kisspeptin
KW - Leptin
UR - http://www.scopus.com/inward/record.url?scp=85045688970&partnerID=8YFLogxK
U2 - 10.12669/pjms.342.14078
DO - 10.12669/pjms.342.14078
M3 - Article
AN - SCOPUS:85045688970
SN - 1682-024X
VL - 34
SP - 342
EP - 346
JO - Pakistan Journal of Medical Sciences
JF - Pakistan Journal of Medical Sciences
IS - 2
ER -