Culture-independent analysis of vaginal microflora: The unrecognized association of Atopobium vaginae with bacterial vaginosis

Hans Verstraelen, Rita Verhelst, Geert Claeys, Marleen Temmerman, Mario Vaneechoutte

Research output: Contribution to journalArticlepeer-review

95 Citations (Scopus)

Abstract

Although the pathogenesis of bacterial vaginosis remains elusive, a few microorganisms, such as Gardnerella vaginalis, are considered markers on Gram stain or culture. Culture-independent analysis of vaginal microflora using 16S rDNA cloning and sequencing of total bacterial communities reveals the gram-positive Atopobium vaginae as a predominant species in disturbed vaginal flora.

Original languageEnglish
Pages (from-to)1130-1132
Number of pages3
JournalAmerican Journal of Obstetrics and Gynecology
Volume191
Issue number4
DOIs
Publication statusPublished - Oct 2004
Externally publishedYes

Keywords

  • 16S rDNA amplification
  • Atopobium vaginae
  • Bacterial vaginosis
  • Vaginal flora

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