Culture-independent analysis of vaginal microflora: The unrecognized association of Atopobium vaginae with bacterial vaginosis

Hans Verstraelen; Rita Verhelst; Geert Claeys; Marleen Temmerman; Mario Vaneechoutte

doi:10.1016/j.ajog.2004.04.013

Culture-independent analysis of vaginal microflora: The unrecognized association of Atopobium vaginae with bacterial vaginosis

Hans Verstraelen
, Rita Verhelst
, Geert Claeys
, Marleen Temmerman
, Mario Vaneechoutte

Research output: Contribution to journal › Article › peer-review

101 Citations (Scopus)

Abstract

Although the pathogenesis of bacterial vaginosis remains elusive, a few microorganisms, such as Gardnerella vaginalis, are considered markers on Gram stain or culture. Culture-independent analysis of vaginal microflora using 16S rDNA cloning and sequencing of total bacterial communities reveals the gram-positive Atopobium vaginae as a predominant species in disturbed vaginal flora.

Original language	English (UK)
Pages (from-to)	1130-1132
Number of pages	3
Journal	American Journal of Obstetrics and Gynecology
Volume	191
Issue number	4
DOIs	https://doi.org/10.1016/j.ajog.2004.04.013
Publication status	Published - Oct 2004
Externally published	Yes

Keywords

16S rDNA amplification
Atopobium vaginae
Bacterial vaginosis
Vaginal flora

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10.1016/j.ajog.2004.04.013

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title = "Culture-independent analysis of vaginal microflora: The unrecognized association of Atopobium vaginae with bacterial vaginosis",

abstract = "Although the pathogenesis of bacterial vaginosis remains elusive, a few microorganisms, such as Gardnerella vaginalis, are considered markers on Gram stain or culture. Culture-independent analysis of vaginal microflora using 16S rDNA cloning and sequencing of total bacterial communities reveals the gram-positive Atopobium vaginae as a predominant species in disturbed vaginal flora.",

keywords = "16S rDNA amplification, Atopobium vaginae, Bacterial vaginosis, Vaginal flora",

author = "Hans Verstraelen and Rita Verhelst and Geert Claeys and Marleen Temmerman and Mario Vaneechoutte",

note = "Funding Information: This work was part of a research project on determinants of differential susceptibility to ascending genital tract infection, and was supported through a research grant by the Marguerite-Marie Delacroix Foundation. As the main funding source, the Marguerite-Marie Delacroix Foundation was not involved in the development of the study design, the collection, analysis, and interpretation of the data, in the writing of the report, or in the decision to submit the paper for publication. H. V. and M. T. participated in the development of the study design, the collection of the study samples, the collection, analysis and interpretation of the data, and in the writing of the report. R. V., G. C., and M. V. participated in the development of the study design, the analysis of the study samples, the collection, analysis, and interpretation of the data, and in the writing of the report.",

year = "2004",

month = oct,

doi = "10.1016/j.ajog.2004.04.013",

language = "English (UK)",

volume = "191",

pages = "1130--1132",

journal = "American Journal of Obstetrics and Gynecology",

issn = "0002-9378",

publisher = "Elsevier Inc.",

number = "4",

}

TY - JOUR

T1 - Culture-independent analysis of vaginal microflora

T2 - The unrecognized association of Atopobium vaginae with bacterial vaginosis

AU - Verstraelen, Hans

AU - Verhelst, Rita

AU - Claeys, Geert

AU - Temmerman, Marleen

AU - Vaneechoutte, Mario

N1 - Funding Information: This work was part of a research project on determinants of differential susceptibility to ascending genital tract infection, and was supported through a research grant by the Marguerite-Marie Delacroix Foundation. As the main funding source, the Marguerite-Marie Delacroix Foundation was not involved in the development of the study design, the collection, analysis, and interpretation of the data, in the writing of the report, or in the decision to submit the paper for publication. H. V. and M. T. participated in the development of the study design, the collection of the study samples, the collection, analysis and interpretation of the data, and in the writing of the report. R. V., G. C., and M. V. participated in the development of the study design, the analysis of the study samples, the collection, analysis, and interpretation of the data, and in the writing of the report.

PY - 2004/10

Y1 - 2004/10

N2 - Although the pathogenesis of bacterial vaginosis remains elusive, a few microorganisms, such as Gardnerella vaginalis, are considered markers on Gram stain or culture. Culture-independent analysis of vaginal microflora using 16S rDNA cloning and sequencing of total bacterial communities reveals the gram-positive Atopobium vaginae as a predominant species in disturbed vaginal flora.

AB - Although the pathogenesis of bacterial vaginosis remains elusive, a few microorganisms, such as Gardnerella vaginalis, are considered markers on Gram stain or culture. Culture-independent analysis of vaginal microflora using 16S rDNA cloning and sequencing of total bacterial communities reveals the gram-positive Atopobium vaginae as a predominant species in disturbed vaginal flora.

KW - 16S rDNA amplification

KW - Atopobium vaginae

KW - Bacterial vaginosis

KW - Vaginal flora

UR - https://www.scopus.com/pages/publications/7044270932

U2 - 10.1016/j.ajog.2004.04.013

DO - 10.1016/j.ajog.2004.04.013

M3 - Article

C2 - 15507931

AN - SCOPUS:7044270932

SN - 0002-9378

VL - 191

SP - 1130

EP - 1132

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

IS - 4

ER -

Culture-independent analysis of vaginal microflora: The unrecognized association of Atopobium vaginae with bacterial vaginosis

Abstract

Keywords

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