Cytokine changes in gastric and colonic epithelial cell in response to Planta ovata extract

Javed Yakoob, Wasim Jafri, Malik Hassan Mehmood, Zaigham Abbas, Kanwal Tariq

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2 Citations (Scopus)


Psyllium (Planta ovata, Ispaghul) seed and husk are used for treatment of altered bowel habit, i.e. constipation and diarrhea. We studied the effect of Ispaghul extract on secretion of interleukin-1 beta (IL-1β) by AGS (ATCC CRL 1739) and SW480 (ATCC CCL-227) epithelial cell lines and determined whether Ispaghul extract has an effect on IL-1β secretion by Helicobacter pylori (H. pylori)-stimulated AGS cell and Escherichia coli K-12 (E. coli K-12)-stimulated SW480 cells in vitro. The AGS cells and SW480 cells were pretreated with Ispaghul extract in concentrations, i.e. 3.5 and 7μg/mL prior to infection with H. pylori and E. coli K-12. DNA fragmentation in AGS and SW480 cells treated with Ispaghul extract was not significant (2.3±0.8?) compared with untreated cells (2.2±0.6?). Ispaghul extract decreased the H. pylori-stimulated secretion of IL-1β by AGS cell (p<0.0001). This effect did not increase as the concentration of extract was increased. Ispaghul extract also decreased E. coli K-12-stimulated IL-1β secretion by SW480 cell (p<0.0001). This effect increased as the concentration of extracts was increased. Ispaghul extract had an effect on stimulated secretion of IL-1β by the AGS and SW480 cell. It decreased pro-inflammatory reaction from both cell lines stimulated by bacteria.

Original languageEnglish
Article number20150075
JournalJournal of Complementary and Integrative Medicine
Issue number2
Publication statusPublished - 16 Mar 2017


  • AGS cell
  • E. coli K-12
  • H. pylori
  • Ispaghul husk
  • SW480
  • interleukin-1β


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