Detection of H2-b and Thy-1.2 Surface Antigens on the Differentiating Murine Otocyst Using Nomarski Optics

MICHAEL B. PRYSTOWSKY; KHALID M. KHAN; WILLIAM F. MAROVITZ

doi:10.1111/j.1432-0436.1979.tb00990.x

Detection of H2-b and Thy-1.2 Surface Antigens on the Differentiating Murine Otocyst Using Nomarski Optics

MICHAEL B. PRYSTOWSKY
, KHALID M. KHAN
, WILLIAM F. MAROVITZ

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The 12th postcoital day otocyst appears as a hollow cellular ball of pseudostratified columnar epithelium that has entered into its initial stages of differentiation and organogenesis. H-2b antigen was demonstrated on the ectodermally derived epithelial cells of the otocysts and on the mesodermally derived cells of the surrounding mesenchyme. Thy-1.2 antigen was detected on the mesenchymal cells, but not on the epithelial cells of the otocyst. The use of Nomarski optics as a new method for detecting cell surface staining that would otherwise be undetected by bright field optics was demonstrated.

Original language	English (UK)
Pages (from-to)	53-58
Number of pages	6
Journal	Differentiation
Volume	12
Issue number	1-3
DOIs	https://doi.org/10.1111/j.1432-0436.1979.tb00990.x
Publication status	Published - 1979
Externally published	Yes

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10.1111/j.1432-0436.1979.tb00990.x

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title = "Detection of H2-b and Thy-1.2 Surface Antigens on the Differentiating Murine Otocyst Using Nomarski Optics",

abstract = "The 12th postcoital day otocyst appears as a hollow cellular ball of pseudostratified columnar epithelium that has entered into its initial stages of differentiation and organogenesis. H-2b antigen was demonstrated on the ectodermally derived epithelial cells of the otocysts and on the mesodermally derived cells of the surrounding mesenchyme. Thy-1.2 antigen was detected on the mesenchymal cells, but not on the epithelial cells of the otocyst. The use of Nomarski optics as a new method for detecting cell surface staining that would otherwise be undetected by bright field optics was demonstrated.",

author = "PRYSTOWSKY, \{MICHAEL B.\} and KHAN, \{KHALID M.\} and MAROVITZ, \{WILLIAM F.\}",

note = "Funding Information: * Sponsored in part by a grant from the Deafness Research Foundation",

year = "1979",

doi = "10.1111/j.1432-0436.1979.tb00990.x",

language = "English (UK)",

volume = "12",

pages = "53--58",

journal = "Differentiation",

issn = "0301-4681",

publisher = "Elsevier Ltd.",

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T1 - Detection of H2-b and Thy-1.2 Surface Antigens on the Differentiating Murine Otocyst Using Nomarski Optics

AU - PRYSTOWSKY, MICHAEL B.

AU - KHAN, KHALID M.

AU - MAROVITZ, WILLIAM F.

N1 - Funding Information: * Sponsored in part by a grant from the Deafness Research Foundation

PY - 1979

Y1 - 1979

N2 - The 12th postcoital day otocyst appears as a hollow cellular ball of pseudostratified columnar epithelium that has entered into its initial stages of differentiation and organogenesis. H-2b antigen was demonstrated on the ectodermally derived epithelial cells of the otocysts and on the mesodermally derived cells of the surrounding mesenchyme. Thy-1.2 antigen was detected on the mesenchymal cells, but not on the epithelial cells of the otocyst. The use of Nomarski optics as a new method for detecting cell surface staining that would otherwise be undetected by bright field optics was demonstrated.

AB - The 12th postcoital day otocyst appears as a hollow cellular ball of pseudostratified columnar epithelium that has entered into its initial stages of differentiation and organogenesis. H-2b antigen was demonstrated on the ectodermally derived epithelial cells of the otocysts and on the mesodermally derived cells of the surrounding mesenchyme. Thy-1.2 antigen was detected on the mesenchymal cells, but not on the epithelial cells of the otocyst. The use of Nomarski optics as a new method for detecting cell surface staining that would otherwise be undetected by bright field optics was demonstrated.

UR - https://www.scopus.com/pages/publications/0018071260

U2 - 10.1111/j.1432-0436.1979.tb00990.x

DO - 10.1111/j.1432-0436.1979.tb00990.x

M3 - Article

C2 - 310405

AN - SCOPUS:0018071260

SN - 0301-4681

VL - 12

SP - 53

EP - 58

JO - Differentiation

JF - Differentiation

IS - 1-3

ER -

Detection of H2-b and Thy-1.2 Surface Antigens on the Differentiating Murine Otocyst Using Nomarski Optics

Abstract

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