TY - JOUR
T1 - Detection of Helicobacter pylori DNA in fecal samples from infected individuals
AU - Gramley, William A.
AU - Asghar, Ali
AU - Frierson, Henry F.
AU - Powell, Steven M.
PY - 1999
Y1 - 1999
N2 - Stool, gastric biopsy, and serum samples were collected from 22 subjects. DNA from stool was extracted, amplified, and hydridized with primers specific for the 16s rRNA gene of Helicobacter pylori. DNA from gastric biopsy specimens was analyzed similarly for comparison. Universal primers were used to confirm successful extraction of DNA from samples. Histologic, serologic, and DNA analyses were scored in a blinded fashion. Universal primer amplification verified successful DNA extraction from all stool and gastric tissue specimens. The gastric tissue DNA assay was positive for H. pylori in 11 of the 22 subjects, correlating completely with histologic and serologic results. Stool DNA was positive for H. pylori by our molecular assay in 8 of these 11 H. pylori-positive subjects. All subjects who were negative by histologic, serologic, and gastric tissue DNA analyses, were also negative by stool DNA analyses. Compared to histology, serology, and gastric tissue DNA analyses, the sensitivity of our stool DNA assay was 73%, with a specificity of 100%.
AB - Stool, gastric biopsy, and serum samples were collected from 22 subjects. DNA from stool was extracted, amplified, and hydridized with primers specific for the 16s rRNA gene of Helicobacter pylori. DNA from gastric biopsy specimens was analyzed similarly for comparison. Universal primers were used to confirm successful extraction of DNA from samples. Histologic, serologic, and DNA analyses were scored in a blinded fashion. Universal primer amplification verified successful DNA extraction from all stool and gastric tissue specimens. The gastric tissue DNA assay was positive for H. pylori in 11 of the 22 subjects, correlating completely with histologic and serologic results. Stool DNA was positive for H. pylori by our molecular assay in 8 of these 11 H. pylori-positive subjects. All subjects who were negative by histologic, serologic, and gastric tissue DNA analyses, were also negative by stool DNA analyses. Compared to histology, serology, and gastric tissue DNA analyses, the sensitivity of our stool DNA assay was 73%, with a specificity of 100%.
UR - https://www.scopus.com/pages/publications/0033039220
U2 - 10.1128/jcm.37.7.2236-2240.1999
DO - 10.1128/jcm.37.7.2236-2240.1999
M3 - Article
C2 - 10364591
AN - SCOPUS:0033039220
SN - 0095-1137
VL - 37
SP - 2236
EP - 2240
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 7
ER -