Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick blood on filter paper from children born to HIV-1 seropositive mothers

P. N. Nyambi; K. Fransen; H. De Beenhouwer; E. N. Chomba; M. Temmerman; J. O. Ndinya- Achola; P. Piot; G. Van der Groen

doi:10.1128/jcm.32.11.2858-2860.1994

Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick blood on filter paper from children born to HIV-1 seropositive mothers

P. N. Nyambi, K. Fransen, H. De Beenhouwer, E. N. Chomba, M. Temmerman, J. O. Ndinya- Achola, P. Piot, G. Van der Groen

Research output: Contribution to journal › Comment/debate

47 Citations (Scopus)

Abstract

The human immunodeficiency virus type 1 (HIV-1) DNA PCR results of 94 dried blood spot (DBS) samples on filter paper and corresponding venous blood in EDTA obtained from infants born to HIV-1-seropositive mothers were compared. In addition, the results of HIV-1 DNA PCR on DBS and the HIV-1 RNA PCR from plasma of 70 paired samples were compared. A 100% specificity and a 95% sensitivity for HIV-1 DNA PCR on DBS compared with results for venous blood were observed for the 94 paired samples. The results of the DBS HIV-1 DNA PCR and HIV-1 RNA PCR of 70 corresponding plasma samples correlated perfectly (100%). The DBS HIV-1 DNA PCR method proved reliable for HIV-1 detection.

Original language	English
Pages (from-to)	2858-2860
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	32
Issue number	11
DOIs	https://doi.org/10.1128/jcm.32.11.2858-2860.1994
Publication status	Published - 1994
Externally published	Yes

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10.1128/jcm.32.11.2858-2860.1994

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Nyambi, P. N., Fransen, K., De Beenhouwer, H., Chomba, E. N., Temmerman, M., Ndinya- Achola, J. O., Piot, P., & Van der Groen, G. (1994). Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick blood on filter paper from children born to HIV-1 seropositive mothers. Journal of Clinical Microbiology, 32(11), 2858-2860. https://doi.org/10.1128/jcm.32.11.2858-2860.1994

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title = "Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick blood on filter paper from children born to HIV-1 seropositive mothers",

abstract = "The human immunodeficiency virus type 1 (HIV-1) DNA PCR results of 94 dried blood spot (DBS) samples on filter paper and corresponding venous blood in EDTA obtained from infants born to HIV-1-seropositive mothers were compared. In addition, the results of HIV-1 DNA PCR on DBS and the HIV-1 RNA PCR from plasma of 70 paired samples were compared. A 100% specificity and a 95% sensitivity for HIV-1 DNA PCR on DBS compared with results for venous blood were observed for the 94 paired samples. The results of the DBS HIV-1 DNA PCR and HIV-1 RNA PCR of 70 corresponding plasma samples correlated perfectly (100%). The DBS HIV-1 DNA PCR method proved reliable for HIV-1 detection.",

author = "Nyambi, {P. N.} and K. Fransen and {De Beenhouwer}, H. and Chomba, {E. N.} and M. Temmerman and {Ndinya- Achola}, {J. O.} and P. Piot and {Van der Groen}, G.",

year = "1994",

doi = "10.1128/jcm.32.11.2858-2860.1994",

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pages = "2858--2860",

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Nyambi, PN, Fransen, K, De Beenhouwer, H, Chomba, EN, Temmerman, M, Ndinya- Achola, JO, Piot, P & Van der Groen, G 1994, 'Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick blood on filter paper from children born to HIV-1 seropositive mothers', Journal of Clinical Microbiology, vol. 32, no. 11, pp. 2858-2860. https://doi.org/10.1128/jcm.32.11.2858-2860.1994

TY - JOUR

T1 - Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick blood on filter paper from children born to HIV-1 seropositive mothers

AU - Nyambi, P. N.

AU - Fransen, K.

AU - De Beenhouwer, H.

AU - Chomba, E. N.

AU - Temmerman, M.

AU - Ndinya- Achola, J. O.

AU - Piot, P.

AU - Van der Groen, G.

PY - 1994

Y1 - 1994

N2 - The human immunodeficiency virus type 1 (HIV-1) DNA PCR results of 94 dried blood spot (DBS) samples on filter paper and corresponding venous blood in EDTA obtained from infants born to HIV-1-seropositive mothers were compared. In addition, the results of HIV-1 DNA PCR on DBS and the HIV-1 RNA PCR from plasma of 70 paired samples were compared. A 100% specificity and a 95% sensitivity for HIV-1 DNA PCR on DBS compared with results for venous blood were observed for the 94 paired samples. The results of the DBS HIV-1 DNA PCR and HIV-1 RNA PCR of 70 corresponding plasma samples correlated perfectly (100%). The DBS HIV-1 DNA PCR method proved reliable for HIV-1 detection.

AB - The human immunodeficiency virus type 1 (HIV-1) DNA PCR results of 94 dried blood spot (DBS) samples on filter paper and corresponding venous blood in EDTA obtained from infants born to HIV-1-seropositive mothers were compared. In addition, the results of HIV-1 DNA PCR on DBS and the HIV-1 RNA PCR from plasma of 70 paired samples were compared. A 100% specificity and a 95% sensitivity for HIV-1 DNA PCR on DBS compared with results for venous blood were observed for the 94 paired samples. The results of the DBS HIV-1 DNA PCR and HIV-1 RNA PCR of 70 corresponding plasma samples correlated perfectly (100%). The DBS HIV-1 DNA PCR method proved reliable for HIV-1 detection.

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U2 - 10.1128/jcm.32.11.2858-2860.1994

DO - 10.1128/jcm.32.11.2858-2860.1994

M3 - Comment/debate

C2 - 7852588

AN - SCOPUS:0028130264

SN - 0095-1137

VL - 32

SP - 2858

EP - 2860

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 11

ER -

Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick blood on filter paper from children born to HIV-1 seropositive mothers

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