DetectionofHumanImmunodeficiencyVirusType1(HIV-1)inHeelPrickBloodonFilterPaperfromChildrenBorntoHIV-1-SeropositiveMothers

P N. Nyambi; K Fransen; H De Beenhouwer; E N. Chomba; Marleen Temmerman; J O Ndinya- Achola; P Piot; G van der Groen

DetectionofHumanImmunodeficiencyVirusType1(HIV-1)inHeelPrickBloodonFilterPaperfromChildrenBorntoHIV-1-SeropositiveMothers

P N. Nyambi, K Fransen, H De Beenhouwer, E N. Chomba, Marleen Temmerman, J O Ndinya- Achola, P Piot, G van der Groen

Centre of Excellence in Women and Child Health, East Africa

Research output: Contribution to journal › Article

Abstract

The human immunodeficiency virus type 1 (HIV-1) DNA PCR results of 94 dried blood spot (DBS) samples on filter paper and corresponding venous blood in EDTA obtained from infants born to HIV-1-seropositive mothers were compared. In addition, the results of HIV-1 DNA PCR on DBS and the HIV-1 RNA PCR from plasma of 70 paired samples were compared. A 100% specificity and a 95% sensitivity for HIV-1 DNA PCR on DBS compared with results for venous blood were observed for the 94 paired samples. The results of the DBS HIV-1 DNA PCR and HIV-1 RNA PCR of 70 corresponding plasma samples correlated perfectly (100%). The DBS HIV-1 DNA PCR method proved reliable for HIV-1 detection.

Original language	Undefined/Unknown
Journal	Faculty of Health Sciences, East Africa
Publication status	Published - 1 Nov 1994

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This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

http://ecommons.aku.edu/eastafrica_fhs_fhs/342

Cite this

@article{7d217ea280104848b381dcc96c7e548c,

title = "DetectionofHumanImmunodeficiencyVirusType1(HIV-1)inHeelPrickBloodonFilterPaperfromChildrenBorntoHIV-1-SeropositiveMothers",

abstract = "The human immunodeficiency virus type 1 (HIV-1) DNA PCR results of 94 dried blood spot (DBS) samples on filter paper and corresponding venous blood in EDTA obtained from infants born to HIV-1-seropositive mothers were compared. In addition, the results of HIV-1 DNA PCR on DBS and the HIV-1 RNA PCR from plasma of 70 paired samples were compared. A 100% specificity and a 95% sensitivity for HIV-1 DNA PCR on DBS compared with results for venous blood were observed for the 94 paired samples. The results of the DBS HIV-1 DNA PCR and HIV-1 RNA PCR of 70 corresponding plasma samples correlated perfectly (100%). The DBS HIV-1 DNA PCR method proved reliable for HIV-1 detection.",

author = "Nyambi, {P N.} and K Fransen and Beenhouwer, {H De} and Chomba, {E N.} and Marleen Temmerman and Achola, {J O Ndinya-} and P Piot and Groen, {G van der}",

year = "1994",

month = nov,

day = "1",

language = "Undefined/Unknown",

journal = "Faculty of Health Sciences, East Africa",

}

TY - JOUR

T1 - DetectionofHumanImmunodeficiencyVirusType1(HIV-1)inHeelPrickBloodonFilterPaperfromChildrenBorntoHIV-1-SeropositiveMothers

AU - Nyambi, P N.

AU - Fransen, K

AU - Beenhouwer, H De

AU - Chomba, E N.

AU - Temmerman, Marleen

AU - Achola, J O Ndinya-

AU - Piot, P

AU - Groen, G van der

PY - 1994/11/1

Y1 - 1994/11/1

N2 - The human immunodeficiency virus type 1 (HIV-1) DNA PCR results of 94 dried blood spot (DBS) samples on filter paper and corresponding venous blood in EDTA obtained from infants born to HIV-1-seropositive mothers were compared. In addition, the results of HIV-1 DNA PCR on DBS and the HIV-1 RNA PCR from plasma of 70 paired samples were compared. A 100% specificity and a 95% sensitivity for HIV-1 DNA PCR on DBS compared with results for venous blood were observed for the 94 paired samples. The results of the DBS HIV-1 DNA PCR and HIV-1 RNA PCR of 70 corresponding plasma samples correlated perfectly (100%). The DBS HIV-1 DNA PCR method proved reliable for HIV-1 detection.

AB - The human immunodeficiency virus type 1 (HIV-1) DNA PCR results of 94 dried blood spot (DBS) samples on filter paper and corresponding venous blood in EDTA obtained from infants born to HIV-1-seropositive mothers were compared. In addition, the results of HIV-1 DNA PCR on DBS and the HIV-1 RNA PCR from plasma of 70 paired samples were compared. A 100% specificity and a 95% sensitivity for HIV-1 DNA PCR on DBS compared with results for venous blood were observed for the 94 paired samples. The results of the DBS HIV-1 DNA PCR and HIV-1 RNA PCR of 70 corresponding plasma samples correlated perfectly (100%). The DBS HIV-1 DNA PCR method proved reliable for HIV-1 detection.

M3 - Article

JO - Faculty of Health Sciences, East Africa

JF - Faculty of Health Sciences, East Africa

ER -