Determination of genetic variation among chickpea genotypes and their F1 crosses with RAPD markers

K. Mahmood; W. Nazeer; M. Naeem; S. Freed; M. A. Khan; G. Abbas; M. Iqbal; A. R. Kulachi; S. A. Sajid; M. A. Sadiq; M. Saleem

doi:10.4238/2013.April.10.1

Determination of genetic variation among chickpea genotypes and their F₁ crosses with RAPD markers

K. Mahmood, W. Nazeer, M. Naeem, S. Freed, M. A. Khan, G. Abbas, M. Iqbal, A. R. Kulachi, S. A. Sajid, M. A. Sadiq, M. Saleem

Research output: Contribution to journal › Article › peer-review

Abstract

The intensity of genetic diversity amongst chickpea genotypes and their crosses is unknown. The current study investigated the genetic diversity of chickpea genotypes and their F₁ crosses by using random amplified polymorphic DNA (RAPD) markers. We assessed the variation among 6 chickpea genotypes and 15 F₁ crosses with the RAPD markers. The 6 parents and their 21 hybrids were carefully studied based on the presence or absence of bands. The level of polymorphism varied with different primers. Of 28 primers used, 21 amplified the genomic DNA in all the varieties, 15 generated polymorphic bands among all the varieties, and 6 produced similar banding patterns.

Original language	English
Pages (from-to)	2127-2137
Number of pages	11
Journal	Genetics and Molecular Research
Volume	12
Issue number	2
DOIs	https://doi.org/10.4238/2013.April.10.1
Publication status	Published - 2013
Externally published	Yes

Keywords

Chickpea
DNA fingerprinting
Genetic diversity
Hybrids
RAPD markers

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10.4238/2013.April.10.1

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title = "Determination of genetic variation among chickpea genotypes and their F1 crosses with RAPD markers",

abstract = "The intensity of genetic diversity amongst chickpea genotypes and their crosses is unknown. The current study investigated the genetic diversity of chickpea genotypes and their F1 crosses by using random amplified polymorphic DNA (RAPD) markers. We assessed the variation among 6 chickpea genotypes and 15 F1 crosses with the RAPD markers. The 6 parents and their 21 hybrids were carefully studied based on the presence or absence of bands. The level of polymorphism varied with different primers. Of 28 primers used, 21 amplified the genomic DNA in all the varieties, 15 generated polymorphic bands among all the varieties, and 6 produced similar banding patterns.",

keywords = "Chickpea, DNA fingerprinting, Genetic diversity, Hybrids, RAPD markers",

author = "K. Mahmood and W. Nazeer and M. Naeem and S. Freed and Khan, {M. A.} and G. Abbas and M. Iqbal and Kulachi, {A. R.} and Sajid, {S. A.} and Sadiq, {M. A.} and M. Saleem",

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doi = "10.4238/2013.April.10.1",

language = "English",

volume = "12",

pages = "2127--2137",

journal = "Genetics and Molecular Research",

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T1 - Determination of genetic variation among chickpea genotypes and their F1 crosses with RAPD markers

AU - Mahmood, K.

AU - Nazeer, W.

AU - Naeem, M.

AU - Freed, S.

AU - Khan, M. A.

AU - Abbas, G.

AU - Iqbal, M.

AU - Kulachi, A. R.

AU - Sajid, S. A.

AU - Sadiq, M. A.

AU - Saleem, M.

PY - 2013

Y1 - 2013

N2 - The intensity of genetic diversity amongst chickpea genotypes and their crosses is unknown. The current study investigated the genetic diversity of chickpea genotypes and their F1 crosses by using random amplified polymorphic DNA (RAPD) markers. We assessed the variation among 6 chickpea genotypes and 15 F1 crosses with the RAPD markers. The 6 parents and their 21 hybrids were carefully studied based on the presence or absence of bands. The level of polymorphism varied with different primers. Of 28 primers used, 21 amplified the genomic DNA in all the varieties, 15 generated polymorphic bands among all the varieties, and 6 produced similar banding patterns.

AB - The intensity of genetic diversity amongst chickpea genotypes and their crosses is unknown. The current study investigated the genetic diversity of chickpea genotypes and their F1 crosses by using random amplified polymorphic DNA (RAPD) markers. We assessed the variation among 6 chickpea genotypes and 15 F1 crosses with the RAPD markers. The 6 parents and their 21 hybrids were carefully studied based on the presence or absence of bands. The level of polymorphism varied with different primers. Of 28 primers used, 21 amplified the genomic DNA in all the varieties, 15 generated polymorphic bands among all the varieties, and 6 produced similar banding patterns.

KW - Chickpea

KW - DNA fingerprinting

KW - Genetic diversity

KW - Hybrids

KW - RAPD markers

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JO - Genetics and Molecular Research

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Determination of genetic variation among chickpea genotypes and their F₁ crosses with RAPD markers

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Keywords

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