TY - JOUR
T1 - DNA-Mediated Nanoscale Metal-Organic Frameworks for Ultrasensitive Photoelectrochemical Enzyme-Free Immunoassay
AU - Zhang, Guangyao
AU - Shan, Dan
AU - Dong, Haifeng
AU - Cosnier, Serge
AU - Al-Ghanim, Khalid Abdullah
AU - Ahmad, Zubair
AU - Mahboob, Shahid
AU - Zhang, Xueji
N1 - Publisher Copyright:
© Copyright 2018 American Chemical Society.
PY - 2018/10/16
Y1 - 2018/10/16
N2 - A novel enzyme-free photoelectrochemical (PEC) immunoassay was developed for the ultrasensitive detection of prostate specific antigen (PSA) based on the DNA-mediated nanoscale zirconium-porphyrin MOFs (NMOFs). By virtue of the intrinsic coordination between unsaturated zirconium sites of the NMOFs frameworks and phosphonate groups, the 5′-phosphorylared ss-DNA-tagged antibody (Ab-DNA) conjugate with a consecutive stretch of guanines as a spacer could be loaded on the NMOFs easily, obtaining a novel type of Ab-DNA-functionalized NMOFs complex. Additionally, as a photocathode PEC active nanomaterial, NMOFs exhibited a significant enhanced photocurrent response with the presence of dopamine under oxygen-containing aqueous media at -0.3 V (vs Ag/AgCl). Furthermore, with the aid of the electrochemical grafting of polyamidoamine (PAMAM) dendrimers functionalized interface, the novel type of Ab-DNA-NMOFs further served as a PEC signal nanoprobe for the ultrasensitive PSA immunoassay. Under optimal conditions, the corresponding immunosensor possessed a wide calibration range of 1 pg mL-1 to 10 ng mL-1 and a limit of detection (LOD) of 0.2 pg mL-1. This present work demonstrated the promising application of DNA-mediated NMOFs in developing highly sensitive, environmentally friendly, and cost-effective PEC biosensors.
AB - A novel enzyme-free photoelectrochemical (PEC) immunoassay was developed for the ultrasensitive detection of prostate specific antigen (PSA) based on the DNA-mediated nanoscale zirconium-porphyrin MOFs (NMOFs). By virtue of the intrinsic coordination between unsaturated zirconium sites of the NMOFs frameworks and phosphonate groups, the 5′-phosphorylared ss-DNA-tagged antibody (Ab-DNA) conjugate with a consecutive stretch of guanines as a spacer could be loaded on the NMOFs easily, obtaining a novel type of Ab-DNA-functionalized NMOFs complex. Additionally, as a photocathode PEC active nanomaterial, NMOFs exhibited a significant enhanced photocurrent response with the presence of dopamine under oxygen-containing aqueous media at -0.3 V (vs Ag/AgCl). Furthermore, with the aid of the electrochemical grafting of polyamidoamine (PAMAM) dendrimers functionalized interface, the novel type of Ab-DNA-NMOFs further served as a PEC signal nanoprobe for the ultrasensitive PSA immunoassay. Under optimal conditions, the corresponding immunosensor possessed a wide calibration range of 1 pg mL-1 to 10 ng mL-1 and a limit of detection (LOD) of 0.2 pg mL-1. This present work demonstrated the promising application of DNA-mediated NMOFs in developing highly sensitive, environmentally friendly, and cost-effective PEC biosensors.
UR - http://www.scopus.com/inward/record.url?scp=85054356701&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.8b03762
DO - 10.1021/acs.analchem.8b03762
M3 - Article
C2 - 30234968
AN - SCOPUS:85054356701
SN - 0003-2700
VL - 90
SP - 12284
EP - 12291
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 20
ER -