Early initiation of insulin attenuates histological and functional changes in the liver of streptozotocin-induced diabetic rats using 99mTc-sulfur colloid functional imaging

Fatma J. Al-Saeedi; Salah Kh Al-Waheeb; Peramaiyan Rajendran; Khalid M. Khan; Moudhi Sadan

doi:10.1080/10799893.2021.1912097

Early initiation of insulin attenuates histological and functional changes in the liver of streptozotocin-induced diabetic rats using ^99mTc-sulfur colloid functional imaging

Fatma J. Al-Saeedi, Salah Kh Al-Waheeb, Peramaiyan Rajendran, Khalid M. Khan, Moudhi Sadan

Research output: Contribution to journal › Article › peer-review

Abstract

This study aimed to investigate the effect of insulin on the reticuloendothelial system (RES) in the liver and spleen in diabetic rats. Sprague Dawley rats were divided into control, diabetic rats (DM) and diabetic rats treated with insulin (IDM) for 2 weeks. Rats were imaged with technetium-99m-sulfur colloid (^99mTc-SC) tracer to determine regional distributions of the tracer for all groups by drawing regions of interest and then obtained the ratios as the cumulative counts of heart, liver, and spleen to the whole body (WB). Liver tissue from sacrificed rats from each group was examined by light and electron microscopy. ^99mTc-SC uptake ratios showed a lower liver to WB uptake ratio in the DM rats compared to both controls and IDM rats. Electron microscopy showed severe vacuolization of the hepatocytes of DM rats. The IDM rats show complete resolution of the vacuolization. The early administration of insulin for 2 weeks to diabetic rats could significantly resolve the phagocytic RES function and histological changes in the liver.

Original language	English
Pages (from-to)	261-267
Number of pages	7
Journal	Journal of Receptor and Signal Transduction Research
Volume	42
Issue number	3
DOIs	https://doi.org/10.1080/10799893.2021.1912097
Publication status	Published - 2022
Externally published	Yes

Keywords

Diabetes
Sprague Dawley rat
histopathology
insulin
phagocytic cells

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1080/10799893.2021.1912097

Cite this

Al-Saeedi, F. J., Al-Waheeb, S. K., Rajendran, P., Khan, K. M., & Sadan, M. (2022). Early initiation of insulin attenuates histological and functional changes in the liver of streptozotocin-induced diabetic rats using ^99mTc-sulfur colloid functional imaging. Journal of Receptor and Signal Transduction Research, 42(3), 261-267. https://doi.org/10.1080/10799893.2021.1912097

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title = "Early initiation of insulin attenuates histological and functional changes in the liver of streptozotocin-induced diabetic rats using 99mTc-sulfur colloid functional imaging",

abstract = "This study aimed to investigate the effect of insulin on the reticuloendothelial system (RES) in the liver and spleen in diabetic rats. Sprague Dawley rats were divided into control, diabetic rats (DM) and diabetic rats treated with insulin (IDM) for 2 weeks. Rats were imaged with technetium-99m-sulfur colloid (99mTc-SC) tracer to determine regional distributions of the tracer for all groups by drawing regions of interest and then obtained the ratios as the cumulative counts of heart, liver, and spleen to the whole body (WB). Liver tissue from sacrificed rats from each group was examined by light and electron microscopy. 99mTc-SC uptake ratios showed a lower liver to WB uptake ratio in the DM rats compared to both controls and IDM rats. Electron microscopy showed severe vacuolization of the hepatocytes of DM rats. The IDM rats show complete resolution of the vacuolization. The early administration of insulin for 2 weeks to diabetic rats could significantly resolve the phagocytic RES function and histological changes in the liver.",

keywords = "Diabetes, Sprague Dawley rat, histopathology, insulin, phagocytic cells",

author = "Al-Saeedi, {Fatma J.} and Al-Waheeb, {Salah Kh} and Peramaiyan Rajendran and Khan, {Khalid M.} and Moudhi Sadan",

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year = "2022",

doi = "10.1080/10799893.2021.1912097",

language = "English",

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Al-Saeedi, FJ, Al-Waheeb, SK, Rajendran, P, Khan, KM & Sadan, M 2022, 'Early initiation of insulin attenuates histological and functional changes in the liver of streptozotocin-induced diabetic rats using ^99mTc-sulfur colloid functional imaging', Journal of Receptor and Signal Transduction Research, vol. 42, no. 3, pp. 261-267. https://doi.org/10.1080/10799893.2021.1912097

Early initiation of insulin attenuates histological and functional changes in the liver of streptozotocin-induced diabetic rats using ^99mTc-sulfur colloid functional imaging. / Al-Saeedi, Fatma J.; Al-Waheeb, Salah Kh; Rajendran, Peramaiyan et al.
In: Journal of Receptor and Signal Transduction Research, Vol. 42, No. 3, 2022, p. 261-267.

Research output: Contribution to journal › Article › peer-review

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N2 - This study aimed to investigate the effect of insulin on the reticuloendothelial system (RES) in the liver and spleen in diabetic rats. Sprague Dawley rats were divided into control, diabetic rats (DM) and diabetic rats treated with insulin (IDM) for 2 weeks. Rats were imaged with technetium-99m-sulfur colloid (99mTc-SC) tracer to determine regional distributions of the tracer for all groups by drawing regions of interest and then obtained the ratios as the cumulative counts of heart, liver, and spleen to the whole body (WB). Liver tissue from sacrificed rats from each group was examined by light and electron microscopy. 99mTc-SC uptake ratios showed a lower liver to WB uptake ratio in the DM rats compared to both controls and IDM rats. Electron microscopy showed severe vacuolization of the hepatocytes of DM rats. The IDM rats show complete resolution of the vacuolization. The early administration of insulin for 2 weeks to diabetic rats could significantly resolve the phagocytic RES function and histological changes in the liver.

AB - This study aimed to investigate the effect of insulin on the reticuloendothelial system (RES) in the liver and spleen in diabetic rats. Sprague Dawley rats were divided into control, diabetic rats (DM) and diabetic rats treated with insulin (IDM) for 2 weeks. Rats were imaged with technetium-99m-sulfur colloid (99mTc-SC) tracer to determine regional distributions of the tracer for all groups by drawing regions of interest and then obtained the ratios as the cumulative counts of heart, liver, and spleen to the whole body (WB). Liver tissue from sacrificed rats from each group was examined by light and electron microscopy. 99mTc-SC uptake ratios showed a lower liver to WB uptake ratio in the DM rats compared to both controls and IDM rats. Electron microscopy showed severe vacuolization of the hepatocytes of DM rats. The IDM rats show complete resolution of the vacuolization. The early administration of insulin for 2 weeks to diabetic rats could significantly resolve the phagocytic RES function and histological changes in the liver.

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