TY - JOUR
T1 - Effects of SOD/catalase mimetic platinum nanoparticles on radiation-induced apoptosis in human lymphoma U937 cells
AU - Jawaid, Paras
AU - Rehman, Mati Ur
AU - Yoshihisa, Yoko
AU - Li, Peng
AU - Zhao, Qing Li
AU - Hassan, Mariame Ali
AU - Miyamoto, Yusei
AU - Shimizu, Tadamichi
AU - Kondo, Takashi
PY - 2014/6
Y1 - 2014/6
N2 - Since polyacrylic acid capped platinum nanoparticles (nano-Pts) are known to have a unique ability to quench superoxide (O2-) and hydrogen peroxide (H2O2), the anti-oxidant activity of nano-Pts against apoptosis induced by x-irradiation in human lymphoma U937 cells was investigated. DNA fragmentation assay, Annexin V-FITC/PI by flow cytometry and Giemsa staining revealed a significant decrease in apoptosis induced by 10 Gy, when cells were pre-treated with nano-Pts in a dose-dependent manner. Pre-treatment with nano-Pts significantly decreased radiation-induced reactive oxygen species (ROS) production, Fas expression and loss of mitochondrial membrane potential as determined by flow-cytometry. Furthermore, western blot analysis also showed that the expression of cleaved caspase-3, Bid and cytosolic cytochrome- c were significantly reduced in nano-Pts pretreated cells. Due to the catalase mimetic activity of nano-Pts, these results indicate that pre-treatment of U937 cells with nano-Pts significantly protect radiation-induced apoptosis by inhibiting intracellular ROS (mainly H 2O2), which plays a key role in the induction of apoptosis, because of no practical observation of intracellular O 2- formation.
AB - Since polyacrylic acid capped platinum nanoparticles (nano-Pts) are known to have a unique ability to quench superoxide (O2-) and hydrogen peroxide (H2O2), the anti-oxidant activity of nano-Pts against apoptosis induced by x-irradiation in human lymphoma U937 cells was investigated. DNA fragmentation assay, Annexin V-FITC/PI by flow cytometry and Giemsa staining revealed a significant decrease in apoptosis induced by 10 Gy, when cells were pre-treated with nano-Pts in a dose-dependent manner. Pre-treatment with nano-Pts significantly decreased radiation-induced reactive oxygen species (ROS) production, Fas expression and loss of mitochondrial membrane potential as determined by flow-cytometry. Furthermore, western blot analysis also showed that the expression of cleaved caspase-3, Bid and cytosolic cytochrome- c were significantly reduced in nano-Pts pretreated cells. Due to the catalase mimetic activity of nano-Pts, these results indicate that pre-treatment of U937 cells with nano-Pts significantly protect radiation-induced apoptosis by inhibiting intracellular ROS (mainly H 2O2), which plays a key role in the induction of apoptosis, because of no practical observation of intracellular O 2- formation.
KW - Apoptosis
KW - Platinum nano-particles
KW - Radiation
KW - Reactive Oxygen Species (ROS)
UR - http://www.scopus.com/inward/record.url?scp=84902359932&partnerID=8YFLogxK
U2 - 10.1007/s10495-014-0972-5
DO - 10.1007/s10495-014-0972-5
M3 - Article
C2 - 24687228
AN - SCOPUS:84902359932
SN - 1360-8185
VL - 19
SP - 1006
EP - 1016
JO - Apoptosis : an international journal on programmed cell death
JF - Apoptosis : an international journal on programmed cell death
IS - 6
ER -
