Effects of SOD/catalase mimetic platinum nanoparticles on radiation-induced apoptosis in human lymphoma U937 cells

Paras Jawaid; Mati Ur Rehman; Yoko Yoshihisa; Peng Li; Qing Li Zhao; Mariame Ali Hassan; Yusei Miyamoto; Tadamichi Shimizu; Takashi Kondo

doi:10.1007/s10495-014-0972-5

Effects of SOD/catalase mimetic platinum nanoparticles on radiation-induced apoptosis in human lymphoma U937 cells

Paras Jawaid
, Mati Ur Rehman
, Yoko Yoshihisa
, Peng Li
, Qing Li Zhao
, Mariame Ali Hassan
, Yusei Miyamoto
, Tadamichi Shimizu
, Takashi Kondo

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

Since polyacrylic acid capped platinum nanoparticles (nano-Pts) are known to have a unique ability to quench superoxide (O₂^-) and hydrogen peroxide (H₂O₂), the anti-oxidant activity of nano-Pts against apoptosis induced by x-irradiation in human lymphoma U937 cells was investigated. DNA fragmentation assay, Annexin V-FITC/PI by flow cytometry and Giemsa staining revealed a significant decrease in apoptosis induced by 10 Gy, when cells were pre-treated with nano-Pts in a dose-dependent manner. Pre-treatment with nano-Pts significantly decreased radiation-induced reactive oxygen species (ROS) production, Fas expression and loss of mitochondrial membrane potential as determined by flow-cytometry. Furthermore, western blot analysis also showed that the expression of cleaved caspase-3, Bid and cytosolic cytochrome- c were significantly reduced in nano-Pts pretreated cells. Due to the catalase mimetic activity of nano-Pts, these results indicate that pre-treatment of U937 cells with nano-Pts significantly protect radiation-induced apoptosis by inhibiting intracellular ROS (mainly H ₂O₂), which plays a key role in the induction of apoptosis, because of no practical observation of intracellular O ₂^- formation.

Original language	English (UK)
Pages (from-to)	1006-1016
Number of pages	11
Journal	Apoptosis : an international journal on programmed cell death
Volume	19
Issue number	6
DOIs	https://doi.org/10.1007/s10495-014-0972-5
Publication status	Published - Jun 2014
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Apoptosis
Platinum nano-particles
Radiation
Reactive Oxygen Species (ROS)

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10.1007/s10495-014-0972-5

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title = "Effects of SOD/catalase mimetic platinum nanoparticles on radiation-induced apoptosis in human lymphoma U937 cells",

abstract = "Since polyacrylic acid capped platinum nanoparticles (nano-Pts) are known to have a unique ability to quench superoxide (O2-) and hydrogen peroxide (H2O2), the anti-oxidant activity of nano-Pts against apoptosis induced by x-irradiation in human lymphoma U937 cells was investigated. DNA fragmentation assay, Annexin V-FITC/PI by flow cytometry and Giemsa staining revealed a significant decrease in apoptosis induced by 10 Gy, when cells were pre-treated with nano-Pts in a dose-dependent manner. Pre-treatment with nano-Pts significantly decreased radiation-induced reactive oxygen species (ROS) production, Fas expression and loss of mitochondrial membrane potential as determined by flow-cytometry. Furthermore, western blot analysis also showed that the expression of cleaved caspase-3, Bid and cytosolic cytochrome- c were significantly reduced in nano-Pts pretreated cells. Due to the catalase mimetic activity of nano-Pts, these results indicate that pre-treatment of U937 cells with nano-Pts significantly protect radiation-induced apoptosis by inhibiting intracellular ROS (mainly H 2O2), which plays a key role in the induction of apoptosis, because of no practical observation of intracellular O 2- formation.",

keywords = "Apoptosis, Platinum nano-particles, Radiation, Reactive Oxygen Species (ROS)",

author = "Paras Jawaid and Rehman, \{Mati Ur\} and Yoko Yoshihisa and Peng Li and Zhao, \{Qing Li\} and Hassan, \{Mariame Ali\} and Yusei Miyamoto and Tadamichi Shimizu and Takashi Kondo",

year = "2014",

month = jun,

doi = "10.1007/s10495-014-0972-5",

language = "English (UK)",

volume = "19",

pages = "1006--1016",

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T1 - Effects of SOD/catalase mimetic platinum nanoparticles on radiation-induced apoptosis in human lymphoma U937 cells

AU - Jawaid, Paras

AU - Rehman, Mati Ur

AU - Yoshihisa, Yoko

AU - Li, Peng

AU - Zhao, Qing Li

AU - Hassan, Mariame Ali

AU - Miyamoto, Yusei

AU - Shimizu, Tadamichi

AU - Kondo, Takashi

PY - 2014/6

Y1 - 2014/6

N2 - Since polyacrylic acid capped platinum nanoparticles (nano-Pts) are known to have a unique ability to quench superoxide (O2-) and hydrogen peroxide (H2O2), the anti-oxidant activity of nano-Pts against apoptosis induced by x-irradiation in human lymphoma U937 cells was investigated. DNA fragmentation assay, Annexin V-FITC/PI by flow cytometry and Giemsa staining revealed a significant decrease in apoptosis induced by 10 Gy, when cells were pre-treated with nano-Pts in a dose-dependent manner. Pre-treatment with nano-Pts significantly decreased radiation-induced reactive oxygen species (ROS) production, Fas expression and loss of mitochondrial membrane potential as determined by flow-cytometry. Furthermore, western blot analysis also showed that the expression of cleaved caspase-3, Bid and cytosolic cytochrome- c were significantly reduced in nano-Pts pretreated cells. Due to the catalase mimetic activity of nano-Pts, these results indicate that pre-treatment of U937 cells with nano-Pts significantly protect radiation-induced apoptosis by inhibiting intracellular ROS (mainly H 2O2), which plays a key role in the induction of apoptosis, because of no practical observation of intracellular O 2- formation.

AB - Since polyacrylic acid capped platinum nanoparticles (nano-Pts) are known to have a unique ability to quench superoxide (O2-) and hydrogen peroxide (H2O2), the anti-oxidant activity of nano-Pts against apoptosis induced by x-irradiation in human lymphoma U937 cells was investigated. DNA fragmentation assay, Annexin V-FITC/PI by flow cytometry and Giemsa staining revealed a significant decrease in apoptosis induced by 10 Gy, when cells were pre-treated with nano-Pts in a dose-dependent manner. Pre-treatment with nano-Pts significantly decreased radiation-induced reactive oxygen species (ROS) production, Fas expression and loss of mitochondrial membrane potential as determined by flow-cytometry. Furthermore, western blot analysis also showed that the expression of cleaved caspase-3, Bid and cytosolic cytochrome- c were significantly reduced in nano-Pts pretreated cells. Due to the catalase mimetic activity of nano-Pts, these results indicate that pre-treatment of U937 cells with nano-Pts significantly protect radiation-induced apoptosis by inhibiting intracellular ROS (mainly H 2O2), which plays a key role in the induction of apoptosis, because of no practical observation of intracellular O 2- formation.

KW - Apoptosis

KW - Platinum nano-particles

KW - Radiation

KW - Reactive Oxygen Species (ROS)

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DO - 10.1007/s10495-014-0972-5

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C2 - 24687228

AN - SCOPUS:84902359932

SN - 1360-8185

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JO - Apoptosis : an international journal on programmed cell death

JF - Apoptosis : an international journal on programmed cell death

IS - 6

ER -

Effects of SOD/catalase mimetic platinum nanoparticles on radiation-induced apoptosis in human lymphoma U937 cells

Abstract

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Keywords

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