Entamoeba species associated with chronic diarrhoea in Pakistan

J. Yakoob; Z. Abbas; M. A. Beg; S. Naz; R. Khan; W. Jafri

doi:10.1017/S0950268811000215

Entamoeba species associated with chronic diarrhoea in Pakistan

J. Yakoob, Z. Abbas, M. A. Beg, S. Naz, R. Khan, W. Jafri

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

We determined the prevalence of Entamoeba (E.) histolytica, E. dispar and E. moshkovskii in patients with chronic diarrhoea associated with abdominal pain or discomfort mimicking irritable bowel syndrome. Stool samples were collected from 161 patients with chronic diarrhoea and from 157 healthy controls. Stool microscopy with modified trichrome stain, culture and polymerase chain reaction (PCR) for Entamoeba spp. differentiation was performed. Microscopy demonstrated Entamoeba cysts in 44% (57/129) of patients with diarrhoea compared to 29% (44/151) of controls (P=0.009). In patients with diarrhoea, PCR for E. histolytica was positive in 9% (11/129) (P=0.008), E. dispar in 19% (24/129) (P=0.117) and E. moshkovskii in 19% (24/129) (P<0.001). E. histolytica and E. moshkovskii were significantly associated with diarrhoea while E. dispar was found equally in both groups.

Original language	English
Pages (from-to)	323-328
Number of pages	6
Journal	Epidemiology and Infection
Volume	140
Issue number	2
DOIs	https://doi.org/10.1017/S0950268811000215
Publication status	Published - Feb 2012

Keywords

Diarrhoea
Entamoeba dispar
Entamoeba histolytica
Entamoeba moshkovskii
PCR

UN SDGs

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10.1017/S0950268811000215

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title = "Entamoeba species associated with chronic diarrhoea in Pakistan",

abstract = "We determined the prevalence of Entamoeba (E.) histolytica, E. dispar and E. moshkovskii in patients with chronic diarrhoea associated with abdominal pain or discomfort mimicking irritable bowel syndrome. Stool samples were collected from 161 patients with chronic diarrhoea and from 157 healthy controls. Stool microscopy with modified trichrome stain, culture and polymerase chain reaction (PCR) for Entamoeba spp. differentiation was performed. Microscopy demonstrated Entamoeba cysts in 44% (57/129) of patients with diarrhoea compared to 29% (44/151) of controls (P=0.009). In patients with diarrhoea, PCR for E. histolytica was positive in 9% (11/129) (P=0.008), E. dispar in 19% (24/129) (P=0.117) and E. moshkovskii in 19% (24/129) (P<0.001). E. histolytica and E. moshkovskii were significantly associated with diarrhoea while E. dispar was found equally in both groups.",

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author = "J. Yakoob and Z. Abbas and Beg, {M. A.} and S. Naz and R. Khan and W. Jafri",

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AU - Yakoob, J.

AU - Abbas, Z.

AU - Beg, M. A.

AU - Naz, S.

AU - Khan, R.

AU - Jafri, W.

PY - 2012/2

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N2 - We determined the prevalence of Entamoeba (E.) histolytica, E. dispar and E. moshkovskii in patients with chronic diarrhoea associated with abdominal pain or discomfort mimicking irritable bowel syndrome. Stool samples were collected from 161 patients with chronic diarrhoea and from 157 healthy controls. Stool microscopy with modified trichrome stain, culture and polymerase chain reaction (PCR) for Entamoeba spp. differentiation was performed. Microscopy demonstrated Entamoeba cysts in 44% (57/129) of patients with diarrhoea compared to 29% (44/151) of controls (P=0.009). In patients with diarrhoea, PCR for E. histolytica was positive in 9% (11/129) (P=0.008), E. dispar in 19% (24/129) (P=0.117) and E. moshkovskii in 19% (24/129) (P<0.001). E. histolytica and E. moshkovskii were significantly associated with diarrhoea while E. dispar was found equally in both groups.

AB - We determined the prevalence of Entamoeba (E.) histolytica, E. dispar and E. moshkovskii in patients with chronic diarrhoea associated with abdominal pain or discomfort mimicking irritable bowel syndrome. Stool samples were collected from 161 patients with chronic diarrhoea and from 157 healthy controls. Stool microscopy with modified trichrome stain, culture and polymerase chain reaction (PCR) for Entamoeba spp. differentiation was performed. Microscopy demonstrated Entamoeba cysts in 44% (57/129) of patients with diarrhoea compared to 29% (44/151) of controls (P=0.009). In patients with diarrhoea, PCR for E. histolytica was positive in 9% (11/129) (P=0.008), E. dispar in 19% (24/129) (P=0.117) and E. moshkovskii in 19% (24/129) (P<0.001). E. histolytica and E. moshkovskii were significantly associated with diarrhoea while E. dispar was found equally in both groups.

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KW - Entamoeba dispar

KW - Entamoeba histolytica

KW - Entamoeba moshkovskii

KW - PCR

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ER -

Entamoeba species associated with chronic diarrhoea in Pakistan

Abstract

Keywords

UN SDGs

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