Enteropathogenic and enteroaggregative E. coli in stools of children with acute gastroenteritis in Davidson County, Tennessee

Monique A. Foster; Junaid Iqbal; Chengxian Zhang; Rendie McHenry; Brent E. Cleveland; Yesenia Romero-Herazo; Chris Fonnesbeck; Daniel C. Payne; James D. Chappell; Natasha Halasa; Oscar G. Gómez-Duarte

doi:10.1016/j.diagmicrobio.2015.07.016

Enteropathogenic and enteroaggregative E. coli in stools of children with acute gastroenteritis in Davidson County, Tennessee

Monique A. Foster, Junaid Iqbal, Chengxian Zhang, Rendie McHenry, Brent E. Cleveland, Yesenia Romero-Herazo, Chris Fonnesbeck, Daniel C. Payne, James D. Chappell, Natasha Halasa, Oscar G. Gómez-Duarte

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

This prospective acute gastroenteritis (AGE) surveillance was conducted in the inpatient and emergency room settings at a referral pediatric hospital to determine the prevalence of diarrheagenic Escherichia coli (DEC) in children <12. years of age with AGE in Davidson County, Tennessee. Subjects 15 days to 11 years of age, who presented with diarrhea and/or vomiting, were enrolled. Stool specimens were processed for detection of DEC using multiplex polymerase chain reaction. From December 1, 2011, to June 30, 2012, a total of 79 (38%) out of 206 stool specimens from children with AGE tested positive for E. coli. A total of 12 (5.8%) out of 206 stool specimens from children with AGE were positive for a DEC. Eight (67%) out of these 12 were positive for enteropathogenic E. coli, and the remaining 4 were positive for enteroaggregative E. coli. DEC clinical isolates clustered with known E. coli enteropathogens according to multilocus sequencing typing.

Original language	English
Pages (from-to)	319-324
Number of pages	6
Journal	Diagnostic Microbiology and Infectious Disease
Volume	83
Issue number	3
DOIs	https://doi.org/10.1016/j.diagmicrobio.2015.07.016
Publication status	Published - Nov 2015
Externally published	Yes

Keywords

Children
Diarrhea
E. coli
EAEC
EPEC
Epidemiology
Gastroenteritis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.diagmicrobio.2015.07.016

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Foster, M. A., Iqbal, J., Zhang, C., McHenry, R., Cleveland, B. E., Romero-Herazo, Y., Fonnesbeck, C., Payne, D. C., Chappell, J. D., Halasa, N., & Gómez-Duarte, O. G. (2015). Enteropathogenic and enteroaggregative E. coli in stools of children with acute gastroenteritis in Davidson County, Tennessee. Diagnostic Microbiology and Infectious Disease, 83(3), 319-324. https://doi.org/10.1016/j.diagmicrobio.2015.07.016

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title = "Enteropathogenic and enteroaggregative E. coli in stools of children with acute gastroenteritis in Davidson County, Tennessee",

abstract = "This prospective acute gastroenteritis (AGE) surveillance was conducted in the inpatient and emergency room settings at a referral pediatric hospital to determine the prevalence of diarrheagenic Escherichia coli (DEC) in children <12. years of age with AGE in Davidson County, Tennessee. Subjects 15 days to 11 years of age, who presented with diarrhea and/or vomiting, were enrolled. Stool specimens were processed for detection of DEC using multiplex polymerase chain reaction. From December 1, 2011, to June 30, 2012, a total of 79 (38%) out of 206 stool specimens from children with AGE tested positive for E. coli. A total of 12 (5.8%) out of 206 stool specimens from children with AGE were positive for a DEC. Eight (67%) out of these 12 were positive for enteropathogenic E. coli, and the remaining 4 were positive for enteroaggregative E. coli. DEC clinical isolates clustered with known E. coli enteropathogens according to multilocus sequencing typing.",

keywords = "Children, Diarrhea, E. coli, EAEC, EPEC, Epidemiology, Gastroenteritis",

author = "Foster, {Monique A.} and Junaid Iqbal and Chengxian Zhang and Rendie McHenry and Cleveland, {Brent E.} and Yesenia Romero-Herazo and Chris Fonnesbeck and Payne, {Daniel C.} and Chappell, {James D.} and Natasha Halasa and G{\'o}mez-Duarte, {Oscar G.}",

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year = "2015",

month = nov,

doi = "10.1016/j.diagmicrobio.2015.07.016",

language = "English",

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Foster, MA, Iqbal, J, Zhang, C, McHenry, R, Cleveland, BE, Romero-Herazo, Y, Fonnesbeck, C, Payne, DC, Chappell, JD, Halasa, N & Gómez-Duarte, OG 2015, 'Enteropathogenic and enteroaggregative E. coli in stools of children with acute gastroenteritis in Davidson County, Tennessee', Diagnostic Microbiology and Infectious Disease, vol. 83, no. 3, pp. 319-324. https://doi.org/10.1016/j.diagmicrobio.2015.07.016

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AU - Foster, Monique A.

AU - Iqbal, Junaid

AU - Zhang, Chengxian

AU - McHenry, Rendie

AU - Cleveland, Brent E.

AU - Romero-Herazo, Yesenia

AU - Fonnesbeck, Chris

AU - Payne, Daniel C.

AU - Chappell, James D.

AU - Halasa, Natasha

AU - Gómez-Duarte, Oscar G.

PY - 2015/11

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N2 - This prospective acute gastroenteritis (AGE) surveillance was conducted in the inpatient and emergency room settings at a referral pediatric hospital to determine the prevalence of diarrheagenic Escherichia coli (DEC) in children <12. years of age with AGE in Davidson County, Tennessee. Subjects 15 days to 11 years of age, who presented with diarrhea and/or vomiting, were enrolled. Stool specimens were processed for detection of DEC using multiplex polymerase chain reaction. From December 1, 2011, to June 30, 2012, a total of 79 (38%) out of 206 stool specimens from children with AGE tested positive for E. coli. A total of 12 (5.8%) out of 206 stool specimens from children with AGE were positive for a DEC. Eight (67%) out of these 12 were positive for enteropathogenic E. coli, and the remaining 4 were positive for enteroaggregative E. coli. DEC clinical isolates clustered with known E. coli enteropathogens according to multilocus sequencing typing.

AB - This prospective acute gastroenteritis (AGE) surveillance was conducted in the inpatient and emergency room settings at a referral pediatric hospital to determine the prevalence of diarrheagenic Escherichia coli (DEC) in children <12. years of age with AGE in Davidson County, Tennessee. Subjects 15 days to 11 years of age, who presented with diarrhea and/or vomiting, were enrolled. Stool specimens were processed for detection of DEC using multiplex polymerase chain reaction. From December 1, 2011, to June 30, 2012, a total of 79 (38%) out of 206 stool specimens from children with AGE tested positive for E. coli. A total of 12 (5.8%) out of 206 stool specimens from children with AGE were positive for a DEC. Eight (67%) out of these 12 were positive for enteropathogenic E. coli, and the remaining 4 were positive for enteroaggregative E. coli. DEC clinical isolates clustered with known E. coli enteropathogens according to multilocus sequencing typing.

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KW - E. coli

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KW - EPEC

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Enteropathogenic and enteroaggregative E. coli in stools of children with acute gastroenteritis in Davidson County, Tennessee

Abstract

Keywords

UN SDGs

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