TY - JOUR
T1 - Establishing reference interval for thyroid-stimulating hormone in children below two-year ages in Pakistani population
AU - Muneer, Siraj
AU - Siddiqui, Imran
AU - Majid, Hafsa
AU - Jafri, Lena
AU - Humayun, Khadija Nuzhat
AU - Ahmed, Sibtain
AU - Khan, Aysha Habib
N1 - Funding Information:
This project was funded by Departmental Resident Research Grant 2018 by Department of Pathology and Laboratory Medicine , Aga Khan University, Karachi Pakistan.
Funding Information:
This project was funded by Departmental Resident Research Grant 2018 by Department of Pathology and Laboratory Medicine, Aga Khan University, Karachi Pakistan.
Publisher Copyright:
© 2021
PY - 2021/8
Y1 - 2021/8
N2 - Introduction: Reference intervals (RIs) of thyroid-stimulating hormone (TSH) and free thyroxine (FT4) are age, assay and population specific. Currently, the age and assay-specific RIs for TSH are not available for children under two years of age. This study aimed to establish reference intervals for serum concentrations of TSH and FT4 in healthy children aged 1–24 months as per CLSI C28-A3 guidelines. Methods: This prospective cross-sectional study was conducted in children from 1 to 24 months visiting the clinical laboratory for serum vitamin D testing but without any recent illness, hospitalization, medication and history of maternal thyroid diseases from August 2018 to March 2019 were invited to participate in the study. Serum TSH and FT4 were measured on ADVIA Centaur (Siemens Diagnostics, US), using chemiluminescence immunoassay. Kolmogorov–Smirnov test assessed normality of the data and RIs based on central 95% of the population were established using the non-parametric approach. Results: After excluding one subject with confirmed congenital hypothyroidism, a total of 131 children were included in the study. The median (IQR) age of the study subjects was 12 months (11), and majority 78 (59.5%) were boys. The RIs were established using non-parametric approach as the data was not normally distributed. Reference interval for TSH was 0.73–4.94 μIU/mL and for FT4 was 0.81–1.51 ng/dl. Conclusion: We established assay-specific RIs for serum TSH and FT4 in children aged 1–24 months in our population. The RIs were slightly lower from RIs developed on other platforms in different population.
AB - Introduction: Reference intervals (RIs) of thyroid-stimulating hormone (TSH) and free thyroxine (FT4) are age, assay and population specific. Currently, the age and assay-specific RIs for TSH are not available for children under two years of age. This study aimed to establish reference intervals for serum concentrations of TSH and FT4 in healthy children aged 1–24 months as per CLSI C28-A3 guidelines. Methods: This prospective cross-sectional study was conducted in children from 1 to 24 months visiting the clinical laboratory for serum vitamin D testing but without any recent illness, hospitalization, medication and history of maternal thyroid diseases from August 2018 to March 2019 were invited to participate in the study. Serum TSH and FT4 were measured on ADVIA Centaur (Siemens Diagnostics, US), using chemiluminescence immunoassay. Kolmogorov–Smirnov test assessed normality of the data and RIs based on central 95% of the population were established using the non-parametric approach. Results: After excluding one subject with confirmed congenital hypothyroidism, a total of 131 children were included in the study. The median (IQR) age of the study subjects was 12 months (11), and majority 78 (59.5%) were boys. The RIs were established using non-parametric approach as the data was not normally distributed. Reference interval for TSH was 0.73–4.94 μIU/mL and for FT4 was 0.81–1.51 ng/dl. Conclusion: We established assay-specific RIs for serum TSH and FT4 in children aged 1–24 months in our population. The RIs were slightly lower from RIs developed on other platforms in different population.
KW - Pakistan
KW - Reference interval
KW - Thyroid stimulating hormone
UR - http://www.scopus.com/inward/record.url?scp=85111598612&partnerID=8YFLogxK
U2 - 10.1016/j.amsu.2021.102601
DO - 10.1016/j.amsu.2021.102601
M3 - Article
AN - SCOPUS:85111598612
SN - 2049-0801
VL - 68
JO - Annals of Medicine and Surgery
JF - Annals of Medicine and Surgery
M1 - 102601
ER -