Establishment of Reference Intervals for Alkaline Phosphatase in Pakistani Children Using a Data Mining Approach

Sibtain Ahmed, Jakob Zierk, Aysha Habib Khan

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Objective: To establish reference intervals (RIs) for alkaline phosphatase (ALP) levels in Pakistani children using an indirect data mining approach. Methods: ALP levels analyzed on a Siemens Advia 1800 analyzer using the International Federation of Clinical Chemistry’s photometric method for both inpatients and outpatients aged 1 to 17 years between January 2013 and December 2017, including patients from intensive care units and specialty units, were retrieved. RIs were calculated using a previously validated indirect algorithm developed by the German Society of Clinical Chemistry and Laboratory Medicine’s Working Group on Guide Limits. Results: From a total of 108,845 results, after the exclusion of patients with multiple specimens, RIs were calculated for 24,628 males and 18,083 females with stratification into fine-grained age groups. These RIs demonstrate the complex age- and sex-related ALP dynamics occurring during physiological development. Conclusion: The population-specific RIs serve to allow an accurate understanding of the fluctuations in analyte activity with increasing age and to support clinical decision making.

Original languageEnglish
Pages (from-to)484-490
Number of pages7
JournalLaboratory Medicine
Issue number5
Publication statusPublished - 2021


  • Alkaline phosphatase
  • Data
  • Laboratory
  • Pakistan
  • Pediatrics
  • Reference intervals


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