TY - JOUR
T1 - Evaluation of germicidal uv-c light for surface disinfection in a tertiary care hospital
AU - Fahim, Qanita
AU - Uddin, Nasir
AU - Ul Hassan, Najam
AU - Sabir, Nargis
AU - Tariq, Anam
AU - Bashir, Ayesha
AU - Ahmad, Sarmad
N1 - Publisher Copyright:
© 2021, Army Medical College. All rights reserved.
PY - 2021/6/30
Y1 - 2021/6/30
N2 - Objective: To check the efficacy of 36-Watt Ultraviolet-C tube light, in terms of distance and time against medically important microorganisms (Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa, Candida albicans and Aspergillus species). Study Design: Quasi-experimental study. Place and Duration of Study: Pathology department, Combined Military Hospital, Lahore Pakistan, from Jun to Sep 2020. Methodology: ATCC control organisms of above mentioned bacteria, yeasts, and fungi were exposed to ultraviolet-C light for different times and distances to ascertain its germicidal effect. Two methods were selected, one in which micro-organisms inoculated plates were exposed to ultraviolet-C light and second in which McFarland suspensions of microorganisms were exposed before inoculation. Both the methods were compared. Observations were noted down after repeated performance of both the procedures. Results: An exposure time of 15 minutes, mean ± SD (13.8 ± 10.1) at 1-foot distance was proved ideal for all the tested bacteria, but yeasts and fungi required >30 minutes, mean ± SD (17.5 ± 13.5) to be killed. Moreover, distance and time of exposure were found out to be directly proportional irrespective of microbial load. Greater the distance longer the ultraviolet C exposure was required. Conclusion: Ultrviolet-C light 36-Watt can have efficient inactivation of bacterial, fungal and archaeal species up to 6 feet for >30 minutes exposure time. Ultraviolet-C light disinfection is best for areas like closed rooms, operation theatres, PCR Labs, and bio-safety cabinets keeping bio-safety guidelines in view.
AB - Objective: To check the efficacy of 36-Watt Ultraviolet-C tube light, in terms of distance and time against medically important microorganisms (Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa, Candida albicans and Aspergillus species). Study Design: Quasi-experimental study. Place and Duration of Study: Pathology department, Combined Military Hospital, Lahore Pakistan, from Jun to Sep 2020. Methodology: ATCC control organisms of above mentioned bacteria, yeasts, and fungi were exposed to ultraviolet-C light for different times and distances to ascertain its germicidal effect. Two methods were selected, one in which micro-organisms inoculated plates were exposed to ultraviolet-C light and second in which McFarland suspensions of microorganisms were exposed before inoculation. Both the methods were compared. Observations were noted down after repeated performance of both the procedures. Results: An exposure time of 15 minutes, mean ± SD (13.8 ± 10.1) at 1-foot distance was proved ideal for all the tested bacteria, but yeasts and fungi required >30 minutes, mean ± SD (17.5 ± 13.5) to be killed. Moreover, distance and time of exposure were found out to be directly proportional irrespective of microbial load. Greater the distance longer the ultraviolet C exposure was required. Conclusion: Ultrviolet-C light 36-Watt can have efficient inactivation of bacterial, fungal and archaeal species up to 6 feet for >30 minutes exposure time. Ultraviolet-C light disinfection is best for areas like closed rooms, operation theatres, PCR Labs, and bio-safety cabinets keeping bio-safety guidelines in view.
KW - Disinfection
KW - Microorganisms
KW - Ultraviolet-C light
UR - http://www.scopus.com/inward/record.url?scp=85118655941&partnerID=8YFLogxK
U2 - 10.51253/pafmj.v71i3.5894
DO - 10.51253/pafmj.v71i3.5894
M3 - Article
AN - SCOPUS:85118655941
SN - 0030-9648
VL - 71
SP - 889
EP - 894
JO - Pakistan Armed Forces Medical Journal
JF - Pakistan Armed Forces Medical Journal
IS - 3
ER -