Gardnerella vaginalis comprises three distinct genotypes of which only two produce sialidase

Guido Lopes Dos Santos Santiago, Pieter Deschaght, Nabil El Aila, Teresa N. Kiama, Hans Verstraelen, Kimberly K. Jefferson, Marleen Temmerman, Mario Vaneechoutte

Research output: Contribution to journalArticlepeer-review

85 Citations (Scopus)

Abstract

Objective: Sialidase and the presence of Gardnerella vaginalis have been proposed as biomarkers for bacterial vaginosis. Sialidase has been associated with adverse pregnancy outcome. We genotyped G vaginalis isolates, assessed the presence and diversity of sialidase-encoding genes, and determined the production of sialidase. Study Design: One hundred thirty-four G vaginalis isolates were genotyped by random amplified polymorphic deoxyribonucleic acid (RAPD) and a selection of 29 isolates with amplified ribosomal deoxyribonucleic acid restriction analysis (ARDRA). A G vaginalis sialidase quantitative polymerase chain reaction was developed, and the sialidase production was assessed with the filter spot test. Results: Three G vaginalis genotypes could be distinguished by both RAPD and ARDRA. Only 2 genotypes encoded and produced sialidase. Conclusion: Three genotypes exist among G vaginalis isolates, and there is a clear link between genotype and sialidase production. A possible link between sialidase production and (symptomatic) bacterial vaginosis and biofilm production can be hypothesized.

Original languageEnglish
Pages (from-to)450.e1-450.e7
JournalAmerican Journal of Obstetrics and Gynecology
Volume204
Issue number5
DOIs
Publication statusPublished - May 2011
Externally publishedYes

Keywords

  • Gardnerella vaginalis
  • adverse pregnancy outcome
  • bacterial vaginosis
  • genotyping
  • sialidase

Fingerprint

Dive into the research topics of 'Gardnerella vaginalis comprises three distinct genotypes of which only two produce sialidase'. Together they form a unique fingerprint.

Cite this