Generation of a human anti-idiotypic antibody that mimics the GD2 antigen

Mansoor N. Saleh, Jamie D. Stapleton, M. B. Khazaeli, Albert F. Lobuglio

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)


In a phase 1 trial, patients with metastatic melanoma received the anti-GD2 murine mAb 14G2a. All patients developed human anti-14G2a antibodies including anti-Id antibodies. Peripheral blood MNCs from one such patient were fused with the murine myeloma cell line Ag8. Four human anti-14G2a secreting hybridomas were generated and the mAb product of one of the hybridomas was characterized. The human mAb 4B5 (hu-IgG A) binds to the variable region of murine 14G2a (anti-Id). The 4B5 binds to the antigen-combining site of 14G2a and inhibits its binding to GD2 expressing Mel-21 cells. Rabbits were immunized with the human anti-Id 485. Sera from the immunized rabbits demonstrated anti-4B5 antibodies and anti-Mel-21 and anti-GD2 reactivity. Furthermore, rabbit sera competitively inhibited binding of 14G2a to Mel-21 cells. Rabbits immunized with 4B5 developed a DTH response when challenged with 4B5 antibody and Mel-21 cells. These studies demonstrate that the human anti-Id 4B5 mimics the GD2 antigen and is capable of eliciting both a humoral and cellular anti-GD2 immune response This antibody could be potentially used as a human anti-Id vaccine in patients with malignant melanoma.

Original languageEnglish
Pages (from-to)3390-3398
Number of pages9
JournalJournal of Immunology
Issue number6
Publication statusPublished - 1993
Externally publishedYes


Dive into the research topics of 'Generation of a human anti-idiotypic antibody that mimics the GD2 antigen'. Together they form a unique fingerprint.

Cite this