Generation of a human Neurochondrin deficient iPSC line KICRi002-A-3 using CRISPR/Cas9

Ambrin Fatima; Jens Schuster; Talia Akram; Maria Sobol; Jan Hoeber; Niklas Dahl

doi:10.1016/j.scr.2020.101758

Generation of a human Neurochondrin deficient iPSC line KICRi002-A-3 using CRISPR/Cas9

Ambrin Fatima, Jens Schuster, Talia Akram, Maria Sobol, Jan Hoeber, Niklas Dahl

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The role of Neurochondrin (NCDN) in humans is not well understood. Mice with a conditional Ncdn knock-out show epileptic seizures, depressive-like behaviours and impaired spatial learning. Using CRISPR/Cas9, we generated a Neurochondrin deficient human iPSC line KICRi002-A-3 carrying a homozygous 752 bp deletion / 2 bp insertion in the NCDN gene. The iPSC line maintained a normal 46,XY karyotype, expressed pluripotency markers and exhibited capability to differentiate into the three germ layers in vitro. Off-target editing was excluded and Neurochondrin expression was not detectable. The iPSC line offers a valuable resource to study the role of Neurochondrin during human neurogenesis.

Original language	English
Article number	101758
Journal	Stem Cell Research
Volume	44
DOIs	https://doi.org/10.1016/j.scr.2020.101758
Publication status	Published - Apr 2020
Externally published	Yes

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title = "Generation of a human Neurochondrin deficient iPSC line KICRi002-A-3 using CRISPR/Cas9",

abstract = "The role of Neurochondrin (NCDN) in humans is not well understood. Mice with a conditional Ncdn knock-out show epileptic seizures, depressive-like behaviours and impaired spatial learning. Using CRISPR/Cas9, we generated a Neurochondrin deficient human iPSC line KICRi002-A-3 carrying a homozygous 752 bp deletion / 2 bp insertion in the NCDN gene. The iPSC line maintained a normal 46,XY karyotype, expressed pluripotency markers and exhibited capability to differentiate into the three germ layers in vitro. Off-target editing was excluded and Neurochondrin expression was not detectable. The iPSC line offers a valuable resource to study the role of Neurochondrin during human neurogenesis.",

author = "Ambrin Fatima and Jens Schuster and Talia Akram and Maria Sobol and Jan Hoeber and Niklas Dahl",

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T1 - Generation of a human Neurochondrin deficient iPSC line KICRi002-A-3 using CRISPR/Cas9

AU - Fatima, Ambrin

AU - Schuster, Jens

AU - Akram, Talia

AU - Sobol, Maria

AU - Hoeber, Jan

AU - Dahl, Niklas

PY - 2020/4

Y1 - 2020/4

N2 - The role of Neurochondrin (NCDN) in humans is not well understood. Mice with a conditional Ncdn knock-out show epileptic seizures, depressive-like behaviours and impaired spatial learning. Using CRISPR/Cas9, we generated a Neurochondrin deficient human iPSC line KICRi002-A-3 carrying a homozygous 752 bp deletion / 2 bp insertion in the NCDN gene. The iPSC line maintained a normal 46,XY karyotype, expressed pluripotency markers and exhibited capability to differentiate into the three germ layers in vitro. Off-target editing was excluded and Neurochondrin expression was not detectable. The iPSC line offers a valuable resource to study the role of Neurochondrin during human neurogenesis.

AB - The role of Neurochondrin (NCDN) in humans is not well understood. Mice with a conditional Ncdn knock-out show epileptic seizures, depressive-like behaviours and impaired spatial learning. Using CRISPR/Cas9, we generated a Neurochondrin deficient human iPSC line KICRi002-A-3 carrying a homozygous 752 bp deletion / 2 bp insertion in the NCDN gene. The iPSC line maintained a normal 46,XY karyotype, expressed pluripotency markers and exhibited capability to differentiate into the three germ layers in vitro. Off-target editing was excluded and Neurochondrin expression was not detectable. The iPSC line offers a valuable resource to study the role of Neurochondrin during human neurogenesis.

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Generation of a human Neurochondrin deficient iPSC line KICRi002-A-3 using CRISPR/Cas9

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