Generation of a ZEB2 deficient human iPSC line (KICRi002A-4)

Jens Schuster; Ambrin Fatima; Natalia Papadopoulos; Claudia de Guidi; Maria Sobol; Niklas Dahl

doi:10.1016/j.scr.2024.103521

Generation of a ZEB2 deficient human iPSC line (KICRi002A-4)

Jens Schuster, Ambrin Fatima, Natalia Papadopoulos, Claudia de Guidi, Maria Sobol, Niklas Dahl

Department of Biological & Biomedical Sciences, Medical College, Pakistan

Research output: Contribution to journal › Article › peer-review

Abstract

The transcription factor ZEB2 is essential for early embryonic development. Using CRISPR/Cas9, we generated a ZEB2 deficient human iPSC cell line (KICRi002A-4), carrying a homozygous 790 bp deletion in ZEB2 that involves part of exon 5, intron 5 and part of exon 6. The deletion leads to markedly reduced levels of a truncated ZEB2 transcript. No ZEB2 protein was detected by immunopreciptation. The iPSC line expressed pluripotency markers and showed a capacity to differentiate into the three germ layers in vitro. Assessment of genomic integrity revealed a normal karyotype without detectable OFF-target editing. The iPSC line KICRi002A-4 thus offers a valuable resource to study the role of ZEB2 for the commitment and differentiation of various human cell lineages.

Original language	English
Article number	103521
Journal	Stem Cell Research
Volume	80
DOIs	https://doi.org/10.1016/j.scr.2024.103521
Publication status	Published - Oct 2024

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title = "Generation of a ZEB2 deficient human iPSC line (KICRi002A-4)",

abstract = "The transcription factor ZEB2 is essential for early embryonic development. Using CRISPR/Cas9, we generated a ZEB2 deficient human iPSC cell line (KICRi002A-4), carrying a homozygous 790 bp deletion in ZEB2 that involves part of exon 5, intron 5 and part of exon 6. The deletion leads to markedly reduced levels of a truncated ZEB2 transcript. No ZEB2 protein was detected by immunopreciptation. The iPSC line expressed pluripotency markers and showed a capacity to differentiate into the three germ layers in vitro. Assessment of genomic integrity revealed a normal karyotype without detectable OFF-target editing. The iPSC line KICRi002A-4 thus offers a valuable resource to study the role of ZEB2 for the commitment and differentiation of various human cell lineages.",

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AU - Schuster, Jens

AU - Fatima, Ambrin

AU - Papadopoulos, Natalia

AU - de Guidi, Claudia

AU - Sobol, Maria

AU - Dahl, Niklas

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AB - The transcription factor ZEB2 is essential for early embryonic development. Using CRISPR/Cas9, we generated a ZEB2 deficient human iPSC cell line (KICRi002A-4), carrying a homozygous 790 bp deletion in ZEB2 that involves part of exon 5, intron 5 and part of exon 6. The deletion leads to markedly reduced levels of a truncated ZEB2 transcript. No ZEB2 protein was detected by immunopreciptation. The iPSC line expressed pluripotency markers and showed a capacity to differentiate into the three germ layers in vitro. Assessment of genomic integrity revealed a normal karyotype without detectable OFF-target editing. The iPSC line KICRi002A-4 thus offers a valuable resource to study the role of ZEB2 for the commitment and differentiation of various human cell lineages.

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Generation of a ZEB2 deficient human iPSC line (KICRi002A-4)

Abstract

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