Genetic and phenotypic analyses of carpel development in Arabidopsis

Vicente Balanzà, Patricia Ballester, Monica Colombo, Chloé Fourquin, Irene Martínez-Fernández, Cristina Ferrándiz

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Citations (Scopus)

Abstract

Carpels are the female reproductive organs of the flower, organized in a gynoecium, which is arguably the most complex organ of a plant. The gynoecium provides protection for the ovules, helps to discriminate between male gametophytes, and facilitates successful pollination. After fertilization, it develops into a fruit, a specialized organ for seed protection and dispersal. To carry out all these functions, coordinated patterning and tissue specification within the developing gynoecium have to be achieved. In this chapter, we describe different methods to characterize defects in carpel patterning and morphogenesis associated with developmental mutations as well as a list of reporter lines that can be used to facilitate genetic analyses.

Original languageEnglish
Title of host publicationFlower Development
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages231-249
Number of pages19
ISBN (Print)9781461494072
DOIs
Publication statusPublished - 2014
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1110
ISSN (Print)1064-3745

Keywords

  • Carpel
  • Cloral hydrate
  • Gynoecium
  • Lignin
  • Pollination
  • Vascular clearing

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