TY - JOUR
T1 - Genome-wide association study identifies variation of glucosidase being linked to natural variation of the maximal quantum yield of photosystem II
AU - Hamdani, Saber
AU - Wang, Hongru
AU - Zheng, Guangyong
AU - Perveen, Shahnaz
AU - Qu, Mingnan
AU - Khan, Naveed
AU - Khan, Waqasuddin
AU - Jiang, Jianjun
AU - Li, Ming
AU - Liu, Xinyu
AU - Zhu, Xiaocen
AU - Govindjee,
AU - Chu, Chengcai
AU - Zhu, Xin Guang
N1 - Funding Information:
The authors would like to thank the Chinese Academy of Sciences Strategic Research Project for financial support. Govindjee would like to thank the Department of Plant Biology, of the University of Illinois at Urbana-Champaign (UIUC) for providing office and Lab space. He gives special thanks to all the members of the staff of Information Technology, School of Integrative Biology and Molecular & Cell Biology, UIUC, for their help with the use of computers.
Funding Information:
Acknowledgement – The authors would like to thank the Chinese Academy of Sciences Strategic Research Project for financial support. Govindjee would like to thank the Department of Plant Biology, of the University of Illinois at Urbana-Champaign (UIUC) for providing office and Lab space. He gives special thanks to all the members of the staff of Information Technology, School of Integrative Biology and Molecular & Cell Biology, UIUC, for their help with the use of computers.
Publisher Copyright:
© 2019 Scandinavian Plant Physiology Society
PY - 2019/5
Y1 - 2019/5
N2 - The maximum quantum yield of photosystem II (as reflected by variable to maximum chlorophyll a fluorescence, F v /F m ) is regarded as one of the most important photosynthetic parameters. The genetic basis underlying natural variation in F v /F m , which shows low level of variations in plants under non-stress conditions, is not easy to be exploited using the conventional gene cloning approaches. Thus, in order to answer this question, we have followed another strategy: we used genome-wide association study (GWAS) and transgenic analysis in a rice mini-core collection. We report here that four single-nucleotide polymorphisms, located in the promoter region of β-glucosidase 5 (BGlu-5), are associated with observed variation in F v /F m . Indeed, our transgenic analysis showed a good correlation between BGlu-5 and F v /F m . Thus, our work demonstrates the feasibility of using GWAS to study natural variation in F v /F m , suggesting that cis-element polymorphism, affecting the BGlu-5 expression level, may, indirectly, contribute to F v /F m variation in rice through the gibberellin signaling pathway. Further research is needed to understand the mechanism of our novel observation.
AB - The maximum quantum yield of photosystem II (as reflected by variable to maximum chlorophyll a fluorescence, F v /F m ) is regarded as one of the most important photosynthetic parameters. The genetic basis underlying natural variation in F v /F m , which shows low level of variations in plants under non-stress conditions, is not easy to be exploited using the conventional gene cloning approaches. Thus, in order to answer this question, we have followed another strategy: we used genome-wide association study (GWAS) and transgenic analysis in a rice mini-core collection. We report here that four single-nucleotide polymorphisms, located in the promoter region of β-glucosidase 5 (BGlu-5), are associated with observed variation in F v /F m . Indeed, our transgenic analysis showed a good correlation between BGlu-5 and F v /F m . Thus, our work demonstrates the feasibility of using GWAS to study natural variation in F v /F m , suggesting that cis-element polymorphism, affecting the BGlu-5 expression level, may, indirectly, contribute to F v /F m variation in rice through the gibberellin signaling pathway. Further research is needed to understand the mechanism of our novel observation.
UR - http://www.scopus.com/inward/record.url?scp=85063499454&partnerID=8YFLogxK
U2 - 10.1111/ppl.12957
DO - 10.1111/ppl.12957
M3 - Article
C2 - 30834537
AN - SCOPUS:85063499454
SN - 0031-9317
VL - 166
SP - 105
EP - 119
JO - Physiologia Plantarum
JF - Physiologia Plantarum
IS - 1
ER -