Genomic diversity of EPEC associated with clinical presentations of differing severity

Tracy H. Hazen, Michael S. Donnenberg, Sandra Panchalingam, Martin Antonio, Anowar Hossain, Inacio Mandomando, John Benjamin Ochieng, Thandavarayan Ramamurthy, Boubou Tamboura, Shahida Qureshi, Farheen Quadri, Anita Zaidi, Karen L. Kotloff, Myron M. Levine, Eileen M. Barry, James B. Kaper, David A. Rasko, James P. Nataro

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)

Abstract

Enteropathogenic Escherichia coli (EPEC) are diarrhoeagenic E. coli, and are a significant cause of gastrointestinal illness among young children in developing countries. Typical EPEC are identified by the presence of the bundle-forming pilus encoded by a virulence plasmid, which has been linked to an increased severity of illness, while atypical EPEC lack this feature. Comparative genomics of 70 total EPEC from lethal (LI), non-lethal symptomatic (NSI) or asymptomatic (AI) cases of diarrhoeal illness in children enrolled in the Global Enteric Multicenter Study was used to investigate the genomic differences in EPEC isolates obtained from individuals with various clinical outcomes. A comparison of the genomes of isolates from different clinical outcomes identified genes that were significantly more prevalent in EPEC isolates of symptomatic and lethal outcomes than in EPEC isolates of asymptomatic outcomes. These EPEC isolates exhibited previously unappreciated phylogenomic diversity and combinations of virulence factors. These comparative results highlight the diversity of the pathogen, as well as the complexity of the EPEC virulence factor repertoire.

Original languageEnglish
Article number15014
JournalNature Microbiology
Volume1
Issue number2
DOIs
Publication statusPublished - 18 Jan 2016

Fingerprint

Dive into the research topics of 'Genomic diversity of EPEC associated with clinical presentations of differing severity'. Together they form a unique fingerprint.

Cite this