Giardia lamblia: Intracellular localization of alpha8-giardin

Chao Jun Wei; Xi Feng Tian; Rodney D. Adam; Si Qi Lu

doi:10.1016/j.exppara.2010.05.028

Giardia lamblia: Intracellular localization of alpha8-giardin

Chao Jun Wei, Xi Feng Tian, Rodney D. Adam, Si Qi Lu

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Alpha8-giardin (α8-giardin) is a member of the multi-gene α-giardin family in the intestinal parasitic protozoan, Giardia lamblia. This gene family shares an ancestry with the annexin super family, whose common characteristic is calcium dependent binding to membranes that contain acidic phospholipids. In the present study, the antigenicity, hydrophilicity, flexibility, surface probability, and secondary structure of α8-giardin amino acids were predicted by bioinformatics applications. A specific anti-peptide antiserum, anti-P3, was used to determine the intracellular location of α8-giardin with confocal immunofluorescence microscopy and immunoelectron microscopy. The results indicated that α8-giardin was located on the plasma membrane and flagella, but not on the ventral disk. Reduction of α8-giardin transcript levels by ribozyme-mediated cleavage decreased trophozoite motility and growth rate, indicating the functional importance of α8-giardin to Giardia trophozoite biology.

Original language	English
Pages (from-to)	489-496
Number of pages	8
Journal	Experimental Parasitology
Volume	126
Issue number	4
DOIs	https://doi.org/10.1016/j.exppara.2010.05.028
Publication status	Published - Dec 2010
Externally published	Yes

Keywords

Annexin
Diplomonad
Giardin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.exppara.2010.05.028

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@article{3890d925de354131969ac092825bdd35,

title = "Giardia lamblia: Intracellular localization of alpha8-giardin",

abstract = "Alpha8-giardin (α8-giardin) is a member of the multi-gene α-giardin family in the intestinal parasitic protozoan, Giardia lamblia. This gene family shares an ancestry with the annexin super family, whose common characteristic is calcium dependent binding to membranes that contain acidic phospholipids. In the present study, the antigenicity, hydrophilicity, flexibility, surface probability, and secondary structure of α8-giardin amino acids were predicted by bioinformatics applications. A specific anti-peptide antiserum, anti-P3, was used to determine the intracellular location of α8-giardin with confocal immunofluorescence microscopy and immunoelectron microscopy. The results indicated that α8-giardin was located on the plasma membrane and flagella, but not on the ventral disk. Reduction of α8-giardin transcript levels by ribozyme-mediated cleavage decreased trophozoite motility and growth rate, indicating the functional importance of α8-giardin to Giardia trophozoite biology.",

keywords = "Annexin, Diplomonad, Giardin",

author = "Wei, {Chao Jun} and Tian, {Xi Feng} and Adam, {Rodney D.} and Lu, {Si Qi}",

note = "Funding Information: This research work was supported by Grant No. 30970313 from the National of Natural Science Foundation of China .",

year = "2010",

month = dec,

doi = "10.1016/j.exppara.2010.05.028",

language = "English",

volume = "126",

pages = "489--496",

journal = "Experimental Parasitology",

issn = "0014-4894",

publisher = "Academic Press Inc.",

number = "4",

}

TY - JOUR

T1 - Giardia lamblia

T2 - Intracellular localization of alpha8-giardin

AU - Wei, Chao Jun

AU - Tian, Xi Feng

AU - Adam, Rodney D.

AU - Lu, Si Qi

N1 - Funding Information: This research work was supported by Grant No. 30970313 from the National of Natural Science Foundation of China .

PY - 2010/12

Y1 - 2010/12

N2 - Alpha8-giardin (α8-giardin) is a member of the multi-gene α-giardin family in the intestinal parasitic protozoan, Giardia lamblia. This gene family shares an ancestry with the annexin super family, whose common characteristic is calcium dependent binding to membranes that contain acidic phospholipids. In the present study, the antigenicity, hydrophilicity, flexibility, surface probability, and secondary structure of α8-giardin amino acids were predicted by bioinformatics applications. A specific anti-peptide antiserum, anti-P3, was used to determine the intracellular location of α8-giardin with confocal immunofluorescence microscopy and immunoelectron microscopy. The results indicated that α8-giardin was located on the plasma membrane and flagella, but not on the ventral disk. Reduction of α8-giardin transcript levels by ribozyme-mediated cleavage decreased trophozoite motility and growth rate, indicating the functional importance of α8-giardin to Giardia trophozoite biology.

AB - Alpha8-giardin (α8-giardin) is a member of the multi-gene α-giardin family in the intestinal parasitic protozoan, Giardia lamblia. This gene family shares an ancestry with the annexin super family, whose common characteristic is calcium dependent binding to membranes that contain acidic phospholipids. In the present study, the antigenicity, hydrophilicity, flexibility, surface probability, and secondary structure of α8-giardin amino acids were predicted by bioinformatics applications. A specific anti-peptide antiserum, anti-P3, was used to determine the intracellular location of α8-giardin with confocal immunofluorescence microscopy and immunoelectron microscopy. The results indicated that α8-giardin was located on the plasma membrane and flagella, but not on the ventral disk. Reduction of α8-giardin transcript levels by ribozyme-mediated cleavage decreased trophozoite motility and growth rate, indicating the functional importance of α8-giardin to Giardia trophozoite biology.

KW - Annexin

KW - Diplomonad

KW - Giardin

UR - http://www.scopus.com/inward/record.url?scp=80054092682&partnerID=8YFLogxK

U2 - 10.1016/j.exppara.2010.05.028

DO - 10.1016/j.exppara.2010.05.028

M3 - Article

C2 - 20515687

AN - SCOPUS:80054092682

SN - 0014-4894

VL - 126

SP - 489

EP - 496

JO - Experimental Parasitology

JF - Experimental Parasitology

IS - 4

ER -

Giardia lamblia: Intracellular localization of alpha8-giardin

Abstract

Keywords

UN SDGs

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