Glutathione s-transferase p deficiency induces glucose intolerance via jnk-dependent enhancement of hepatic gluconeogenesis

Shubha Ghosh Dastidar, Ganapathy Jagatheesan, Petra Haberzettl, Jasmit Shah, Bradford G. Hill, Aruni Bhatnagar, Daniel J. Conklin

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Hepatic glutathione S-transferases (GSTs) are dysregulated in human obesity, nonalcoholic fatty liver disease, and diabetes. The multifunctional GST pi-isoform (GSTP) catalyzes the conjugation of glutathione with acrolein and inhibits c-Jun NH2-terminal kinase (JNK) activation. Herein, we tested whether GSTP deficiency disturbs glucose homeostasis in mice. Hepatic GST proteins were downregulated by short-term high-fat diet in wild-type (WT) mice concomitant with increased glucose intolerance, JNK activation, and cytokine mRNAs in the liver. Genetic deletion of GSTP did not affect body composition, fasting blood glucose levels, or insulin levels in mice maintained on a normal chow diet; however, compared with WT mice, the GSTP-null mice were glucose intolerant. In GSTP-null mice, pyruvate intolerance, reflecting increased hepatic gluconeogenesis, was accompanied by elevated levels of activated JNK, cytokine mRNAs, and glucose-6-phosphatase proteins in the liver. Treatment of GSTP-null mice with the JNK inhibitor 1,9-pyrazoloanthrone (SP600125) significantly attenuated pyruvate-induced hepatic gluconeogenesis and significantly altered correlations between hepatic cytokine mRNAs and metabolic outcomes in GSTP-null mice. Collectively, these findings suggest that hepatic GSTP plays a pivotal role in glucose handling by regulating JNK-dependent control of hepatic gluconeogenesis. Thus, hepatic GSTP-JNK dys-regulation may be a target of new therapeutic interventions during early stages of glucose intolerance to prevent the worsening metabolic derangements associated with human obesity and its relentless progression to diabetes.

Original languageEnglish
Pages (from-to)E1005-E1018
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Issue number5
Publication statusPublished - Nov 2018


  • Gluconeogenesis
  • Glucose intolerance
  • JNK
  • Obesity
  • Type 2 diabetes


Dive into the research topics of 'Glutathione s-transferase p deficiency induces glucose intolerance via jnk-dependent enhancement of hepatic gluconeogenesis'. Together they form a unique fingerprint.

Cite this