Highly sensitive electrochemical detection of living cells based on diamond microelectrode arrays

Jun Ruan; Wenbo Zhang; Hang Zhang; Yun Chen; Fawad Ur Rehman; Hui Jiang; Steffen Strehle; Alberto Pasquarelli; Xuemei Wang

doi:10.1016/j.cclet.2017.10.037

Highly sensitive electrochemical detection of living cells based on diamond microelectrode arrays

Jun Ruan, Wenbo Zhang, Hang Zhang, Yun Chen, Fawad Ur Rehman, Hui Jiang, Steffen Strehle, Alberto Pasquarelli, Xuemei Wang

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We designed boron-doped nanocrystalline diamond microelectrode arrays (BNCD-MEAs) with 16 channels for the bioanalysis of multicellular samples, which could be readily adapted for a highly sensitive detection of H₂O₂ released from stimulated cells by ascorbic acid (AA). Our observations demonstrated that the as-prepared diamond microelectrode arrays could be utilized to distinguish cancer cells from normal cells, and the amperometric study showed the considerable differences in the currents, indicating that the related HepG2 cancer cells could release more H₂O₂ than that of L02 normal cells. This supports the possibility to use diamond-based MEAs for rapid cancer cell detection in future clinic applications.

Original language	English (UK)
Pages (from-to)	919-921
Number of pages	3
Journal	Chinese Chemical Letters
Volume	29
Issue number	6
DOIs	https://doi.org/10.1016/j.cclet.2017.10.037
Publication status	Published - Jun 2018
Externally published	Yes

Keywords

16 Channels
Bioanalysis
BNCD-MEAs
HO detection
Stimulated cells

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.cclet.2017.10.037

Cite this

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title = "Highly sensitive electrochemical detection of living cells based on diamond microelectrode arrays",

abstract = "We designed boron-doped nanocrystalline diamond microelectrode arrays (BNCD-MEAs) with 16 channels for the bioanalysis of multicellular samples, which could be readily adapted for a highly sensitive detection of H2O2 released from stimulated cells by ascorbic acid (AA). Our observations demonstrated that the as-prepared diamond microelectrode arrays could be utilized to distinguish cancer cells from normal cells, and the amperometric study showed the considerable differences in the currents, indicating that the related HepG2 cancer cells could release more H2O2 than that of L02 normal cells. This supports the possibility to use diamond-based MEAs for rapid cancer cell detection in future clinic applications.",

keywords = "16 Channels, Bioanalysis, BNCD-MEAs, HO detection, Stimulated cells",

author = "Jun Ruan and Wenbo Zhang and Hang Zhang and Yun Chen and Rehman, \{Fawad Ur\} and Hui Jiang and Steffen Strehle and Alberto Pasquarelli and Xuemei Wang",

note = "Publisher Copyright: {\textcopyright} 2018 The Author",

year = "2018",

month = jun,

doi = "10.1016/j.cclet.2017.10.037",

language = "English (UK)",

volume = "29",

pages = "919--921",

journal = "Chinese Chemical Letters",

issn = "1001-8417",

publisher = "Elsevier BV",

number = "6",

}

TY - JOUR

T1 - Highly sensitive electrochemical detection of living cells based on diamond microelectrode arrays

AU - Ruan, Jun

AU - Zhang, Wenbo

AU - Zhang, Hang

AU - Chen, Yun

AU - Rehman, Fawad Ur

AU - Jiang, Hui

AU - Strehle, Steffen

AU - Pasquarelli, Alberto

AU - Wang, Xuemei

PY - 2018/6

Y1 - 2018/6

N2 - We designed boron-doped nanocrystalline diamond microelectrode arrays (BNCD-MEAs) with 16 channels for the bioanalysis of multicellular samples, which could be readily adapted for a highly sensitive detection of H2O2 released from stimulated cells by ascorbic acid (AA). Our observations demonstrated that the as-prepared diamond microelectrode arrays could be utilized to distinguish cancer cells from normal cells, and the amperometric study showed the considerable differences in the currents, indicating that the related HepG2 cancer cells could release more H2O2 than that of L02 normal cells. This supports the possibility to use diamond-based MEAs for rapid cancer cell detection in future clinic applications.

AB - We designed boron-doped nanocrystalline diamond microelectrode arrays (BNCD-MEAs) with 16 channels for the bioanalysis of multicellular samples, which could be readily adapted for a highly sensitive detection of H2O2 released from stimulated cells by ascorbic acid (AA). Our observations demonstrated that the as-prepared diamond microelectrode arrays could be utilized to distinguish cancer cells from normal cells, and the amperometric study showed the considerable differences in the currents, indicating that the related HepG2 cancer cells could release more H2O2 than that of L02 normal cells. This supports the possibility to use diamond-based MEAs for rapid cancer cell detection in future clinic applications.

KW - 16 Channels

KW - Bioanalysis

KW - BNCD-MEAs

KW - HO detection

KW - Stimulated cells

UR - https://www.scopus.com/pages/publications/85032788307

U2 - 10.1016/j.cclet.2017.10.037

DO - 10.1016/j.cclet.2017.10.037

M3 - Article

AN - SCOPUS:85032788307

SN - 1001-8417

VL - 29

SP - 919

EP - 921

JO - Chinese Chemical Letters

JF - Chinese Chemical Letters

IS - 6

ER -

Highly sensitive electrochemical detection of living cells based on diamond microelectrode arrays

Abstract

Keywords

UN SDGs

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