TY - JOUR
T1 - Hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) from Pakistan
T2 - molecular characterisation by microarray technology
AU - Jamil, Bushra
AU - Gawlik, Darius
AU - Syed, Muhammad Ali
AU - Shah, Asim Ali
AU - Abbasi, Shahid Ahmad
AU - Müller, Elke
AU - Reißig, Annett
AU - Ehricht, Ralf
AU - Monecke, Stefan
N1 - Funding Information:
Acknowledgements Muhammad Ali Syed would like to acknowledge the Vice Chancellor and the Office of Research, Innovation and Commercialization (ORIC) of the University of Haripur for the financial support.
Funding Information:
Funding information A part of the study was supported by the Vice Chancellor and the Office of Research, Innovation and Commercialization (ORIC) of the University of Haripur, Pakistan. The participating institutions covered the costs of experiments performed in the respective institutions and granted the time needed to perform this study. There was no external funding for this work.
Publisher Copyright:
© 2017, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2018/4/1
Y1 - 2018/4/1
N2 - The prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Pakistan is known to be high, but very few studies have described the molecular epidemiology of the different MRSA clones circulating in the country. Forty-four MRSA isolates were collected from two tertiary care hospitals of the Rawalpindi district of Pakistan. All strains were identified by a conventional phenotypic method and then subjected to genotyping by microarray hybridisation. Six clonal complexes (CCs) and 19 strains were identified. The most commonly identified strains were: (i) Panton–Valentine leucocidin (PVL)-positive CC772-MRSA-V, “Bengal Bay Clone” (ten isolates; 22.3%), (ii) ST239-MRSA [III + ccrC] (five isolates) and (iii) a CC8-MRSA-IV strain, as well as CC6-MRSA-IV (both with four isolates; 9.1% each). Several of the strains detected indicated epidemiological links to the Middle Eastern/Arabian Gulf region. Further studies are needed to type MRSA from countries with less known epidemiology and to monitor the distribution and spread of strains, as well as possible links to global travel, migration and commerce.
AB - The prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Pakistan is known to be high, but very few studies have described the molecular epidemiology of the different MRSA clones circulating in the country. Forty-four MRSA isolates were collected from two tertiary care hospitals of the Rawalpindi district of Pakistan. All strains were identified by a conventional phenotypic method and then subjected to genotyping by microarray hybridisation. Six clonal complexes (CCs) and 19 strains were identified. The most commonly identified strains were: (i) Panton–Valentine leucocidin (PVL)-positive CC772-MRSA-V, “Bengal Bay Clone” (ten isolates; 22.3%), (ii) ST239-MRSA [III + ccrC] (five isolates) and (iii) a CC8-MRSA-IV strain, as well as CC6-MRSA-IV (both with four isolates; 9.1% each). Several of the strains detected indicated epidemiological links to the Middle Eastern/Arabian Gulf region. Further studies are needed to type MRSA from countries with less known epidemiology and to monitor the distribution and spread of strains, as well as possible links to global travel, migration and commerce.
UR - http://www.scopus.com/inward/record.url?scp=85037972418&partnerID=8YFLogxK
U2 - 10.1007/s10096-017-3161-y
DO - 10.1007/s10096-017-3161-y
M3 - Article
C2 - 29238933
AN - SCOPUS:85037972418
SN - 0934-9723
VL - 37
SP - 691
EP - 700
JO - European Journal of Clinical Microbiology and Infectious Diseases
JF - European Journal of Clinical Microbiology and Infectious Diseases
IS - 4
ER -
