Identification of the nucleoli of Giardia lamblia with TEM and CFM

Xi Feng Tian, Zhi Hong Yang, Haie Shen, R. D. Adam, Si Qi Lu

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Giardia species are flagellated parasites of vertebrates and belong to the diplomonads, most of which have two nuclei. These organisms were classified among the earliest branching eukaryotes on the basis of small subunit rDNA sequences and their lack of many canonical organelles. However, some of these organelles have subsequently been identified in rudimentary form, such as Golgi that become apparent during encystation. One of the "missing" organelles has been the nucleolus, the site of rRNA synthesis, since it was not identified in earlier ultrastructural studies. In the current study, we visualized invitro-grown Giardia lamblia trophozoites by transmission electron microscopy and laser scanning confocal microscopy. We found that each of the two nuclei contains a single small and deeply stained granular nucleolus, thus demonstrating that Giardia does indeed have nucleoli.

Original languageEnglish
Pages (from-to)789-793
Number of pages5
JournalParasitology Research
Issue number4
Publication statusPublished - Mar 2010
Externally publishedYes


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