TY - JOUR
T1 - II. Ultrastructural development of the early rat otocyst
AU - Marovitz, W. F.
AU - Khan, K. M.
AU - Schulte, T.
PY - 1977
Y1 - 1977
N2 - Various methods of dissection, fixation, osmication, sectioning and staining were tested in order to develop an acceptable technique for preparing 9, 10, 11, and 12 day old rat otocysts for electron microscopic study. The general problems associated with embryonic tissue difficult handling, high water content and poor stainability are discussed, and concrete methods of preparation which significantly decrease these diffculties are proposed. The specific fixation and sectioning requirements of rat otocyst are also described in the elaboration of a method which will be used in subsequent studies of the organogenesis of rodent ear.
AB - Various methods of dissection, fixation, osmication, sectioning and staining were tested in order to develop an acceptable technique for preparing 9, 10, 11, and 12 day old rat otocysts for electron microscopic study. The general problems associated with embryonic tissue difficult handling, high water content and poor stainability are discussed, and concrete methods of preparation which significantly decrease these diffculties are proposed. The specific fixation and sectioning requirements of rat otocyst are also described in the elaboration of a method which will be used in subsequent studies of the organogenesis of rodent ear.
UR - http://www.scopus.com/inward/record.url?scp=0017367249&partnerID=8YFLogxK
U2 - 10.1177/00034894770860s102
DO - 10.1177/00034894770860s102
M3 - Article
C2 - 402881
AN - SCOPUS:0017367249
SN - 0003-4894
VL - 86
SP - 9
EP - 28
JO - Annals of Otology, Rhinology and Laryngology
JF - Annals of Otology, Rhinology and Laryngology
IS - sup.35
ER -