Immunomodulatory Effects of Psyllium Extract on Helicobacter pylori Interaction With Gastric Epithelial Cells

Javed Yakoob, Wasim Jafri, Malik Hassan Mehmood, Zaigham Abbas, Kanwal Tariq

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Natural plant product Psyllium has anti-inflammatory activity that can modulate the function of cytokines. We determined the effect of Psyllium husk extract on interleukin (IL)-8 and NF-κB secretion by gastric epithelial cells in response to Helicobacter pylori. Human gastric adenocarcinoma cell line (AGS) cells were pretreated with Psyllium extract in different concentrations before H pylori infection. Cell culture supernatant was analyzed for IL-8 and NF-κB by ELISA. RNA from cells was used for real-time polymerase chain reaction for messenger RNA expression of IL-8. Psyllium extract 5 and 10 μg/mL markedly (P <.001) lowered basal IL-8 by 64.71% and 74.51%, respectively, and H pylori–stimulated IL-8 was also (P <.001) lowered by 41.67% and 66.67%, respectively. Psyllium 5 and 10 μg/mL also reduced (P <.0001) cagA-positive H pylori–induced IL-8 mRNA expression by 42.3% and 67.6%, respectively. Psyllium also reduced (P =.0001) NF-κB in response to H pylori strains confirming its role as an anti-inflammatory agent.

Original languageEnglish
Pages (from-to)NP18-NP24
JournalJournal of Evidence-Based Complementary and Alternative Medicine
Volume21
Issue number4
DOIs
Publication statusPublished - 1 Oct 2016

Keywords

  • Helicobacter pylori
  • NF-κB
  • gastric epithelial cells
  • interleukin-8
  • psyllium

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