Natural plant product Psyllium has anti-inflammatory activity that can modulate the function of cytokines. We determined the effect of Psyllium husk extract on interleukin (IL)-8 and NF-κB secretion by gastric epithelial cells in response to Helicobacter pylori. Human gastric adenocarcinoma cell line (AGS) cells were pretreated with Psyllium extract in different concentrations before H pylori infection. Cell culture supernatant was analyzed for IL-8 and NF-κB by ELISA. RNA from cells was used for real-time polymerase chain reaction for messenger RNA expression of IL-8. Psyllium extract 5 and 10 μg/mL markedly (P <.001) lowered basal IL-8 by 64.71% and 74.51%, respectively, and H pylori–stimulated IL-8 was also (P <.001) lowered by 41.67% and 66.67%, respectively. Psyllium 5 and 10 μg/mL also reduced (P <.0001) cagA-positive H pylori–induced IL-8 mRNA expression by 42.3% and 67.6%, respectively. Psyllium also reduced (P =.0001) NF-κB in response to H pylori strains confirming its role as an anti-inflammatory agent.
|Journal||Journal of Evidence-Based Complementary and Alternative Medicine|
|Publication status||Published - 1 Oct 2016|
- Helicobacter pylori
- gastric epithelial cells