L-asparaginase, acrylamide quenching enzyme production from leaves of tamarindus indica and seeds of vigna radiata–fabaceae

Current study aims at high yield production of L-Asparaginase from family fabaceae. Leaves of Tamarindus indica, seeds of Vicia faba, Phaseolus vulgaris, Pisum sativum, Cicer arientinum, Vigna radiata are screened for L-Asparaginase yield and activity. Prepared crude extracts are purified through ammonium sulfate precipitation, dialysis, ion exchange chromatography and gel filtration. When incubated with L-Asparagine, crude extracts of Pisum sativum leaves and seeds of Cicer arientinum, Phaseolus vulgaris and Vicia faba extracts showed 24.93 U/mL, 10.38 U/mL, 11.81U/mL enzymatic activity, respectively. Crude extract of Tamarindus indica leaves exhibited highest enzyme activity of 64.38 U/mL, with a yield of 67% while Vigna radiata seeds crude extract revealed 53% yield with enzymatic activity of 56.34 U/mL.L-Asparaginase enzyme purified from Tamarindus indica has activity of 54.260±0.847 U/mL with specific activity of 95.67 U/mg. L-Asparaginase purified from Vigna radiata seeds exhibit 45.140±0.302 U/mL activity and specific activity 135.25 U/mg. The purified enzyme exhibits maximal activity at 37℃, stability at optimal pH 8.6 in the presence of alkaline 0.2 M potassium phosphate buffer. Optimal substrate concentration for L-Asparaginase activity is 3.5 ug/mL from Tamarindus indica. Results shows that Vigna radiata and Tamarindus indica can be a better choice as high source of L-Asparaginase that can be employed to reduce acrylamide cancer provoking element in food industry.