Ligand binding radioassay for the antifolate compounds: Application in patients receiving methotrexate

S. P. Rothenberg; M. Da Costa; M. Perwaiz Iqbal

Ligand binding radioassay for the antifolate compounds: Application in patients receiving methotrexate

S. P. Rothenberg, M. Da Costa, M. Perwaiz Iqbal

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The general principles of ligand-binding radioassays are reviewed and a specific noncompetitive system is described for the measurement of methotrexate (MTX) using dihydrofolate reductase as the binding determinant. This type of radioassay can also be exploited to measure any antifolate compound which binds to this enzyme even if [³H]MTX is used as the tracer. The radioassay has now been used to measure MTX in all types of body fluids, tissue extracts, and rbc lysates from patients receiving this drug.

Original language	English
Pages (from-to)	575-584
Number of pages	10
Journal	Cancer Treatment Reports
Volume	61
Issue number	4
Publication status	Published - 1977
Externally published	Yes

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Cite this

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title = "Ligand binding radioassay for the antifolate compounds: Application in patients receiving methotrexate",

abstract = "The general principles of ligand-binding radioassays are reviewed and a specific noncompetitive system is described for the measurement of methotrexate (MTX) using dihydrofolate reductase as the binding determinant. This type of radioassay can also be exploited to measure any antifolate compound which binds to this enzyme even if [3H]MTX is used as the tracer. The radioassay has now been used to measure MTX in all types of body fluids, tissue extracts, and rbc lysates from patients receiving this drug.",

author = "Rothenberg, {S. P.} and {Da Costa}, M. and {Perwaiz Iqbal}, M.",

year = "1977",

language = "English",

volume = "61",

pages = "575--584",

journal = "Cancer Treatment Reports",

issn = "0361-5960",

publisher = "Oxford University Press",

number = "4",

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TY - JOUR

T1 - Ligand binding radioassay for the antifolate compounds

T2 - Application in patients receiving methotrexate

AU - Rothenberg, S. P.

AU - Da Costa, M.

AU - Perwaiz Iqbal, M.

PY - 1977

Y1 - 1977

N2 - The general principles of ligand-binding radioassays are reviewed and a specific noncompetitive system is described for the measurement of methotrexate (MTX) using dihydrofolate reductase as the binding determinant. This type of radioassay can also be exploited to measure any antifolate compound which binds to this enzyme even if [3H]MTX is used as the tracer. The radioassay has now been used to measure MTX in all types of body fluids, tissue extracts, and rbc lysates from patients receiving this drug.

AB - The general principles of ligand-binding radioassays are reviewed and a specific noncompetitive system is described for the measurement of methotrexate (MTX) using dihydrofolate reductase as the binding determinant. This type of radioassay can also be exploited to measure any antifolate compound which binds to this enzyme even if [3H]MTX is used as the tracer. The radioassay has now been used to measure MTX in all types of body fluids, tissue extracts, and rbc lysates from patients receiving this drug.

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M3 - Article

C2 - 884692

AN - SCOPUS:0017645122

SN - 0361-5960

VL - 61

SP - 575

EP - 584

JO - Cancer Treatment Reports

JF - Cancer Treatment Reports

IS - 4

ER -

Ligand binding radioassay for the antifolate compounds: Application in patients receiving methotrexate

Abstract

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